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HR6B/UBE2B Antibody

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Western Blot: HR6B/UBE2B Antibody [NBP2-20781] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at ...read more
Western Blot: HR6B/UBE2B Antibody [NBP2-20781] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody diluted at 1:500. The signal was developed with Trident ECL ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Rb, RM, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HR6B/UBE2B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HR6B/UBE2B. The exact sequence is proprietary.
Localization
Cell membrane (By similarity); Nucleus (By similarity)
Predicted Species
Rat (100%), Porcine (100%), Rabbit (100%), Bovine (100%), Rhesus Macaque (100%), Zebrafish (95%), Xenopus (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBE2B
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for HR6B/UBE2B Antibody

  • E2 protein
  • E2-17k
  • E2-17kDa
  • HHR6B
  • HR6B
  • RAD6 homolog B
  • RAD6BEC 6.3.2.19
  • UBC2
  • UBE2B
  • Ubiquitin carrier protein B
  • ubiquitin-conjugating enzyme E2 B
  • Ubiquitin-conjugating enzyme E2-17 kDa
  • ubiquitin-conjugating enzyme E2B (RAD6 homolog)
  • Ubiquitin-protein ligase B

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol UBE2B
Uniprot