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HR6B/UBE2B Antibody (SR1269)

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Western Blot: HR6B/UBE2B Antibody (SR1269) [NBP3-22417] - Western blot analysis using NBP3-22417 in Jurkat cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR1269
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HR6B/UBE2B Antibody (SR1269) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from HR6B/UBE2B (Uniprot #: P63146)
Specificity
Detects endogenous levels of total HR6B/UBE2B
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
UBE2B
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for HR6B/UBE2B Antibody (SR1269)

  • E2 protein
  • E2-17k
  • E2-17kDa
  • HHR6B
  • HR6B
  • RAD6 homolog B
  • RAD6BEC 6.3.2.19
  • UBC2
  • UBE2B
  • Ubiquitin carrier protein B
  • ubiquitin-conjugating enzyme E2 B
  • Ubiquitin-conjugating enzyme E2-17 kDa
  • ubiquitin-conjugating enzyme E2B (RAD6 homolog)
  • Ubiquitin-protein ligase B

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE2B