![Western Blot: HOOK2 Antibody [NBP2-14986] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/HOOK2-Antibody-Western-Blot-NBP2-14986-img0004.jpg "Western Blot: HOOK2 Antibody [NBP2-14986] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE; antibody diluted at 1:1000.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HOOK2. The exact sequence is proprietary. |
| Predicted Species | Rat (90%), Bovine (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HOOK2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 83 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: HOOK2 Antibody [NBP2-14986] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HOOK2 protein stained by HOOK2 antibody (NBP2-14986) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HOOK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14986-img0010.jpg "Immunocytochemistry/Immunofluorescence: HOOK2 Antibody [NBP2-14986] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HOOK2 protein stained by HOOK2 antibody (NBP2-14986) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-43837) diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: HOOK2 Antibody [NBP2-14986] - Paraffin-embedded mouse testis.HOOK2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HOOK2-Antibody-Immunohistochemistry-Paraffin-NBP2-14986-img0012.jpg "Immunohistochemistry-Paraffin: HOOK2 Antibody [NBP2-14986] - Paraffin-embedded mouse testis.HOOK2 antibody diluted at 1:500.")
![Western Blot: HOOK2 Antibody [NBP2-14986] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/HOOK2-Antibody-Western-Blot-NBP2-14986-img0003.jpg "Western Blot: HOOK2 Antibody [NBP2-14986] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: HOOK2 Antibody [NBP2-14986] - Paraffin-embedded mouse duodenum. HOOK2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HOOK2-Antibody-Immunohistochemistry-Paraffin-NBP2-14986-img0011.jpg "Immunohistochemistry-Paraffin: HOOK2 Antibody [NBP2-14986] - Paraffin-embedded mouse duodenum. HOOK2 antibody diluted at 1:500.")
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![Intracellular Staining by Flow Cytometry Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated]](https://images.novusbio.com/images/antibody/mab1266_human-serpin-a5-mab-clone-210326-intracellular-staining-by-flow-cytometry-662020192248.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")