Home » HO-1/HMOX1/HSP32 » HO-1/HMOX1/HSP32 Antibodies » HO-1/HMOX1/HSP32 Antibody (412811) [Unconjugated]
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HO-1/HMOX1/HSP32 Antibody (412811) [Unconjugated]

 (7 Publications)
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Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, KO
Clone
412811
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3776-SP
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HO-1/HMOX1/HSP32 Antibody (412811) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HO-1/HMOX1
Met1-Thr261
Accession # P09601
Specificity
Detects endogenous human and mouse HO-1 in Western blots. In Western blots, this antibody does not cross‑react with rhHO-2.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
HMOX1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB3776 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HO-1/HMOX1/HSP32 Antibody (412811) [Unconjugated]

  • bK286B10
  • EC 1.14.99.3
  • heat shock protein, 32-kD
  • heme oxygenase (decycling) 1
  • HMOX1
  • HO
  • HO1
  • HO-1
  • HO-1heme oxygenase 1
  • HSP32

Background

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, an inducible Heme Oxygenase-1 (HO-1/HMOX1) and a constitutive Heme Oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HO-1/HMOX1/HSP32 Antibody (MAB3776)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Xenograft.

We have publications tested in 2 applications: IHC, Western Blot.


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(1)
Western Blot
(3)
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(1)
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(2)
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(1)
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Showing Publications 1 - 7 of 7.
Publications using MAB3776 Applications Species
Del Vecchio, V;Mele, L;Panda, SK;Sanchez-Pajares, IR;Mosca, L;Tirino, V;Barbieri, M;Bruzzese, F;Luciano, A;Marino, FZ;Accardo, M;Nicoletti, GF;Papaccio, G;Barbieri, A;Desiderio, V; ?2-AR inhibition enhances EGFR antibody efficacy hampering the oxidative stress response machinery Cell death & disease 2023-09-19 [PMID: 37723219] (IHC, Xenograft) IHC Xenograft
GV Ganesh, KM Ramkumar Dysregulation of Nrf2 redox pathway in macrophages under diabetic microenvironment Experimental gerontology, 2021-07-10;152(0):111479. 2021-07-10 [PMID: 34256115] (Western Blot, Mouse) Western Blot Mouse
J Sotty, J Kluza, C De Sousa, M Tardivel, S Anthérieu, LY Alleman, L Canivet, E Perdrix, A Loyens, P Marchetti, JM Lo Guidice, G Garçon Mitochondrial alterations triggered by repeated exposure to fine (PM2.5-0.18) and quasi-ultrafine (PM0.18) fractions of ambient particulate matter Environ Int, 2020-06-22;142(0):105830. 2020-06-22 [PMID: 32585499] (Western Blot, Human) Western Blot Human
Olivia May, Nicolas S. Merle, Anne Grunenwald, Viviane Gnemmi, Juliette Leon, Cloé Payet et al. Heme Drives Susceptibility of Glomerular Endothelium to Complement Overactivation Due to Inefficient Upregulation of Heme Oxygenase-1 Frontiers in Immunology 12/20/2018 [PMID: 30619356]
Tiffany G. Baker, Soumen Roy, Carlene S. Brandon, Inga K. Kramarenko, Shimon P. Francis, Mona Taleb et al. Heat Shock Protein-Mediated Protection Against Cisplatin-Induced Hair Cell Death Journal of the Association for Research in Otolaryngology 2/1/2015 [PMID: 25261194]
Lindsey A. May, Inga I. Kramarenko, Carlene S. Brandon, Christina Voelkel-Johnson, Soumen Roy, Kristy Truong et al. Inner ear supporting cells protect hair cells by secreting HSP70 Journal of Clinical Investigation 8/1/2013 [PMID: 23863716]
Dimitrov JD, Dasgupta S, Navarrete AM, Delignat S, Repesse Y, Meslier Y, Planchais C, Teyssandier M, Motterlini R, Bayry J, Kaveri SV, Lacroix-Desmazes S Induction of heme oxygenase-1 in factor VIII-deficient mice reduces the immune response to therapeutic factor VIII. Blood, 2009-11-04;115(13):2682-5. 2009-11-04 [PMID: 19890094] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol HMOX1
Uniprot