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HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free

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Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Analysis of FOXA1 (3B3NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly. Image using the standard format of this product.
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Analysis of FOXA1 (3B3NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly. Image using the standard format of this product.
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - FOXA1 (3B3NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X ...read more
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Analysis of FoxA1 in human prostate. Image using the standard format of this product.
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Staining of LnCAP cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - (1) Whole cell protein from PC3 and (2) MCF7 was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunohistochemistry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Analysis of FoxA1 in human breast adenocarcinoma. Image using the standard format of this product.
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide Free [NBP2-45355] - Staining of HepG2 cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with the negative control (blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
3B3NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-45354AF350
Alexa Fluor 405
NBP2-45354AF405
Alexa Fluor 488
NBP2-45354AF488
Alexa Fluor 532
NBP2-45354AF532
Alexa Fluor 594
NBP2-45354AF594
Alexa Fluor 647
NBP2-45354AF647
Alexa Fluor 700
NBP2-45354AF700
Alexa Fluor 750
NBP2-45354AF750
Allophycocyanin
NBP2-45354APC
Biotin
NBP2-45354B
CoraFluor 1
NBP2-45354CL1
DyLight 350
NBP2-45354UV
DyLight 405
NBP2-45354V
DyLight 488
NBP2-45354G
DyLight 550
NBP2-45354R
DyLight 594
NBP2-45354DL594
DyLight 650
NBP2-45354C
DyLight 680
NBP2-45354FR
DyLight 755
NBP2-45354IR
FITC
NBP2-45354F
HRP
NBP2-45354H
Janelia Fluor 525
NBP2-45354JF525
Janelia Fluor 549
NBP2-45354JF549
Janelia Fluor 585
NBP2-45354JF585
Janelia Fluor 635
NBP2-45354JF635
Janelia Fluor 646
NBP2-45354JF646
Janelia Fluor 669
NBP2-45354JF669
PE
NBP2-45354PE
PerCP
NBP2-45354PCP
Unconjugated
NBP2-45354
mFluor Violet 450 SE
NBP2-45354MFV450
mFluor Violet 500 SE
NBP2-45354MFV500
mFluor Violet 610 SE
NBP2-45354MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45354
BSA Free
NBP2-45354SS
BSA Free
NBP2-45354
Azide and BSA Free
NBP2-45355SS
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HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free Summary

Immunogen
Partial recombinant human FOXA1 protein (between amino acids 350-473) [UniProt# P55317].
Predicted Species
Mouse (98%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FOXA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 25 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:25 - 1:50
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-4 ug/ml

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (78%)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Background

FoxA1 (forkhead box A1), also called HNF-3a (hepatocyte nuclear factor 3 alpha) or TCF3A (transcription factor 3A) is a 473 amino acid (aa), 49 kDa (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93% and 86% aa identity with mouse and rat FoxA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HNF-3 alpha/FoxA1 Antibody (3B3NB) - Azide and BSA Free
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Bioinformatics

Gene Symbol FOXA1