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HMGCL Antibody (4F4-D1)

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Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - Analysis of HMGCL expression in transfected 293T cell line by HMGCL monoclonal antibody (M01), clone 4F4-D1.Lane 1: HMGCL transfected lysate(34.4 KDa).Lane 2: ...read more
Western Blot: HMGCL Antibody (4F4-D1) [H00003155-M01] - GCL monoclonal antibody (M01), clone 4F4-D1 Western Blot analysis of HMGCL expression in A-431 ( Cat # L015V1 ).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4F4-D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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HMGCL Antibody (4F4-D1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
HMGCL (AAH10570, 1 a.a. ~ 325 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL
Specificity
HMGCL - 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (hydroxymethylglutaricaciduria)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HMGCL
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Publications
Read Publications using H00003155-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HMGCL Antibody (4F4-D1)

  • 3-hydroxy-3-methylglutaryl-CoA lyase
  • 3-hydroxymethyl-3-methylglutaryl-CoA lyase
  • EC 4.1.3.4
  • HLmitochondrial

Background

The protein encoded by the HMGCL gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes thefinal step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associatedwith HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been foundfor this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HMGCL Antibody (H00003155-M01)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HMGCL Antibody (H00003155-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMGCL
Entrez
OMIM
Uniprot