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HMGB2 Antibody (S02-5I5)

Images

 
Western Blot: HMGB2 Antibody (S02-5I5) [NBP3-19876] - Western blot detection of HMGB2 in CHO-K1, Ramos cell lysates using NBP3-19876 (1:1000 diluted). Predicted band size: 24kDa. Observed band size: 24kDa.
Immunohistochemistry-Paraffin: HMGB2 Antibody (S02-5I5) [NBP3-19876] - Immunohistochemistry of HMGB2 in paraffin-embedded Human tonsil using NBP3-19876 at dilution 1/50

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
S02-5I5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.3 mg/ml

Order Details

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HMGB2 Antibody (S02-5I5) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide of human HMGB2 (Uniprot # P26583)
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
HMGB2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
0.3 mg/ml
Purity
Affinity purified

Alternate Names for HMGB2 Antibody (S02-5I5)

  • High mobility group protein 2
  • high mobility group protein B2
  • high-mobility group box 2
  • HMG-2
  • HMG2high-mobility group (nonhistone chromosomal) protein 2

Background

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HMGB2