HMGB2 Antibodies
We offer HMGB2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each HMGB2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HMGB2 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the HMGB2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HMGB2 polyclonal antibodies and browse our HMGB2 monoclonal antibody catalog.
Alternate Names for HMGB2 Antibodies
anti-HMGB2 antibody, anti-High mobility group protein 2 antibody, anti-high mobility group protein B2 antibody, anti-high-mobility group box 2 antibody, anti-HMG-2 antibody, anti-HMG2high-mobility group (nonhistone chromosomal) protein 2 antibody
10 Results for "hmgb2" in Primary Antibodies
![Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of extracts of various cell lines, using HMGB2 Rabbit mAb (NBP3-16774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Western-Blot-NBP3-16774-img0007.jpg "Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of extracts of various cell lines, using HMGB2 Rabbit mAb (NBP3-16774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunofluorescence analysis of U-2 OS cells using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunocytochemistry-Immunofluorescence-NBP3-16774-img0006.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry of paraffin-embedded mouse testis using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunohistochemistry-Paraffin-NBP3-16774-img0003.jpg)
![Chromatin Immunoprecipitation: HMGB2 Antibody (8P10V5) [NBP3-16774] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using HMGB2 antibody (NBP3-16774) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Chromatin-Immunoprecipitation-NBP3-16774-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunofluorescence analysis of C6 cells using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunocytochemistry-Immunofluorescence-NBP3-16774-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunofluorescence analysis of NIH-3T3 cells using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunocytochemistry-Immunofluorescence-NBP3-16774-img0005.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774] - Rat testis using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunohistochemistry-Paraffin-NBP3-16774-img0001.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774] - Human colon using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunohistochemistry-Paraffin-NBP3-16774-img0002.jpg)
![Immunoprecipitation: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of 300ug extracts of Mouse testis cells using 3ug HMGB2 antibody (NBP3-16774). Western blot was performed from the immunoprecipitate using HMGB2 (NBP3-16774) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HMGB2-Antibody-8P10V5-Immunoprecipitation-NBP3-16774-img0008.jpg)
![Western Blot: HMGB2 Antibody [NBP2-38694] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Western-Blot-NBP2-38694-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: HMGB2 Antibody [NBP2-38694] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HMGB2 Antibody [NBP2-38694] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38694-img0009.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunohistochemistry-Paraffin-NBP2-38694-img0016.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Analysis in human bone marrow and skeletal muscle tissues using NBP2-38694 antibody. Corresponding HMGB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunohistochemistry-Paraffin-NBP2-38694-img0011.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunohistochemistry-Paraffin-NBP2-38694-img0012.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunohistochemistry-Paraffin-NBP2-38694-img0013.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunohistochemistry-Paraffin-NBP2-38694-img0014.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP2-38694] - Staining of human colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunohistochemistry-Paraffin-NBP2-38694-img0015.jpg)
![Western Blot: HMGB2 Antibody (JE52-82) [NBP3-32436] - Western blot analysis of HMGB2 on different lysates with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: K-562 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7:Mouse testis tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 24kDa Observed band size: 28 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32436) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-278202415242681.jpg "Western Blot: HMGB2 Antibody (JE52-82) [NBP3-32436] - Western blot analysis of HMGB2 on different lysates with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: K-562 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7:Mouse testis tissue lysate Cell lysates/proteins at 20 ug/Lane. Tissue lysates/proteins at 30 ug/Lane. Predicted band size: 24kDa Observed band size: 28 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32436) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HMGB2 Antibody (JE52-82) [NBP3-32436] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32436) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-298202414304166.jpg)
![Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (JE52-82) [NBP3-32436] - Immunocytochemistry analysis of MCF7 cells labeling HMGB2 with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HMGB2 antibody (NBP3-32436) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-29202410334041.jpg)
![Flow Cytometry: HMGB2 Antibody (JE52-82) [NBP3-32436] - Flow cytometric analysis of MCF7 cells labeling HMGB2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32436, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32436_rabbit-hmgb2-mab-je52-82-29202416453289.jpg)
![Western Blot: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HMGB2-Antibody-3E5-Western-Blot-H00003148-M05-img0007.jpg "Western Blot: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of monoclonal antibody to HMGB2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HMGB2-Antibody-3E5-Immunocytochemistry-Immunofluorescence-H00003148-M05-img0005.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody (3E5) [H00003148-M05] - Analysis of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HMGB2-Antibody-3E5-Immunohistochemistry-Paraffin-H00003148-M05-img0006.jpg)
![ELISA: HMGB2 Antibody (3E5) [H00003148-M05] - Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMGB2-Antibody-3E5-ELISA-H00003148-M05-img0004.jpg)
![Western Blot: HMGB2 Antibody (S02-5I5) [NBP3-19876] - Western blot detection of HMGB2 in CHO-K1, Ramos cell lysates using NBP3-19876 (1:1000 diluted). Predicted band size: 24kDa. Observed band size: 24kDa.](http://images.novusbio.com/fullsize/HMGB2-Antibody-S02-5I5-Western-Blot-NBP3-19876-img0002.jpg "Western Blot: HMGB2 Antibody (S02-5I5) [NBP3-19876] - Western blot detection of HMGB2 in CHO-K1, Ramos cell lysates using NBP3-19876 (1:1000 diluted). Predicted band size: 24kDa. Observed band size: 24kDa.")
![Immunohistochemistry-Paraffin: HMGB2 Antibody (S02-5I5) [NBP3-19876] - Immunohistochemistry of HMGB2 in paraffin-embedded Human tonsil using NBP3-19876 at dilution 1/50](http://images.novusbio.com/fullsize/HMGB2-Antibody-S02-5I5-Immunohistochemistry-Paraffin-NBP3-19876-img0001.jpg)
![Western Blot: HMGB2 Antibody (X1) [H00003148-M07] - Analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HMGB2-Antibody-X1-Western-Blot-H00003148-M07-img0004.jpg "Western Blot: HMGB2 Antibody (X1) [H00003148-M07] - Analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (X1) [H00003148-M07] - Analysis of monoclonal antibody to HMGB2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HMGB2-Antibody-X1-Immunocytochemistry-Immunofluorescence-H00003148-M07-img0002.jpg)
![Sandwich ELISA: HMGB2 Antibody (X1) [H00003148-M07] - Detection limit for recombinant GST tagged HMGB2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMGB2-Antibody-X1-Sandwich-ELISA-H00003148-M07-img0003.jpg)
![Western Blot: HMGB2 Antibody (4G7) [H00003148-M02] - Analysis of HMGB2 expression in transfected 293T cell line by HMGB2 monoclonal antibody (M02), clone 4G7.Lane 1: HMGB2 transfected lysate(22 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HMGB2-Antibody-4G7-Western-Blot-H00003148-M02-img0008.jpg "Western Blot: HMGB2 Antibody (4G7) [H00003148-M02] - Analysis of HMGB2 expression in transfected 293T cell line by HMGB2 monoclonal antibody (M02), clone 4G7.Lane 1: HMGB2 transfected lysate(22 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: HMGB2 Antibody (4G7) [H00003148-M02] - HMGB2 monoclonal antibody (M02), clone 4G7. Analysis of HMGB2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/HMGB2-Antibody-4G7-Western-Blot-H00003148-M02-img0006.jpg)
![Western Blot: HMGB2 Antibody (4G7) [H00003148-M02] - HMGB2 monoclonal antibody (M02), clone 4G7 Analysis of HMGB2 expression in K-562.](http://images.novusbio.com/fullsize/HMGB2-Antibody-4G7-Western-Blot-H00003148-M02-img0007.jpg)
![Sandwich ELISA: HMGB2 Antibody (4G7) [H00003148-M02] - Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMGB2-Antibody-4G7-Sandwich-ELISA-H00003148-M02-img0005.jpg)
![Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35743_rabbit-hmgb2-pab-912202415171171.jpg "Western Blot: HMGB2 Antibody [NBP3-35743] - Western blot analysis of various lysates using HMGB2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: HMGB2 Antibody (3F2) [H00003148-M06] - HMGB2 monoclonal antibody (M06), clone 3F2. Western Blot analysis of HMGB2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/HMGB2-Antibody-3F2-Western-Blot-H00003148-M06-img0003.jpg "Western Blot: HMGB2 Antibody (3F2) [H00003148-M06] - HMGB2 monoclonal antibody (M06), clone 3F2. Western Blot analysis of HMGB2 expression in Hela S3 NE.")
![Western Blot: HMGB2 Antibody (3F2) [H00003148-M06] - HMGB2 monoclonal antibody (M06), clone 3F2 Western Blot analysis of HMGB2 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/HMGB2-Antibody-3F2-Western-Blot-H00003148-M06-img0001.jpg)
![Sandwich ELISA: HMGB2 Antibody (3F2) [H00003148-M06] - Detection limit for recombinant GST tagged HMGB2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMGB2-Antibody-3F2-Sandwich-ELISA-H00003148-M06-img0002.jpg)
![Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP1-81731] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/HMGB2-Antibody-Immunohistochemistry-Paraffin-NBP1-81731-img0007.jpg "Immunohistochemistry-Paraffin: HMGB2 Antibody [NBP1-81731] - Staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.")