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HLA C Antibody (DT-9) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IP, CyTOF-ready
Clone
DT-9
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

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HLA C Antibody (DT-9) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Purified tamarin MHC class I molecules (using anti-class I antibody BB7.7)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HLA-C
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for HLA C Antibody (DT-9) [CoraFluor™ 1]

  • C alpha chain
  • D6S204
  • FLJ27082
  • HLA class I histocompatibility antigen, Cw-1 alpha chain
  • HLAC
  • HLA-C
  • HLA-Cw
  • HLA-JY3
  • HLC-C
  • human leukocyte antigen-C alpha chain
  • leucocyte antigen C
  • leucocyte antigen Cw
  • leukocyte antigen-C alpha chain
  • major histocompatibility antigen HLA-C
  • major histocompatibility complex, class I, C
  • MHC class I antigen Cw*1
  • MHC class I antigen Cw*12
  • MHC class I antigen Cw*14
  • MHC class I antigen Cw*15
  • MHC class I antigen Cw*16
  • MHC class I antigen Cw*17
  • MHC class I antigen Cw*2
  • MHC class I antigen Cw*3
  • MHC class I antigen Cw*4
  • MHC class I antigen Cw*5
  • MHC class I antigen Cw*6
  • MHC class I antigen Cw*7
  • MHC class I antigen Cw*8
  • MHC class I antigen heavy chain HLA-C
  • PSORS1

Background

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HLA-C