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Histone H4 [ac Lys16] Antibody - BSA Free

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Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, MaSpecies Glossary
Applications WB, DB, ICC/IF, ChIP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.62 mg/ml

Order Details

Shipping $45.00 per order
Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Histone H4 [ac Lys16] Antibody - BSA Free Summary

Immunogen
Synthetic acetylated peptide surrounding Lysine 16 of human Histone H4 [Swiss Prot P62805].
Modification
ac Lys16
Localization
Nucleus. Chromosome.
Marker
H4k16ac
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H4C16
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Dot Blot 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
Application Notes
This H4K16ac antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Dot Blot. In Western Blot, a band is seen ~13kDa in HeLa histone prep and NIH 3T3 histone prep. In ICC/IF, nuclear staining was observed in HeLa cells. This antibody is not applicable for Chromatin Immunoprecipitation.
Reviewed Applications
Read 1 Review rated 5
using
NB21-2077 in the following applications:

Publications
Read Publications using
NB21-2077 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted to react with most mammal species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.62 mg/ml
Purity
Immunogen affinity purified

Notes

EpiPlus antibodies are produced in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H4 [ac Lys16] Antibody - BSA Free

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4K16ac
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. In particular, acetylation of H4 Lys16 (H4 K16Ac) plays a role in transcriptional activation and DNA repair. The protein MOF (MYST1) is the primary enzyme to acetylate K16, along with Gcn5, Esal, ATF2, and Sas2. Depletion of MOF reduces K16 acetylation, causing cell cycle arrests, chromosomal aberrations, and a poor DNA damage response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Verified Customer
11/17/2016
Application: WB
Species: Pig

Bioinformatics

Gene Symbol H4C16
Entrez
Uniprot