| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2024AF405 | |||
| Alexa Fluor 488 | NB21-2024AF488 | |||
| Alexa Fluor 647 | NB21-2024AF647 | |||
| Alexa Fluor 700 | NB21-2024AF700 | |||
| Biotin | NB21-2024B | |||
| DyLight 405 | NB21-2024V | |||
| DyLight 488 | NB21-2024G | |||
| DyLight 550 | NB21-2024R | |||
| DyLight 650 | NB21-2024C | |||
| FITC | NB21-2024F | |||
| HRP | NB21-2024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2024SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2077AF405 | |||
| Alexa Fluor 488 | NB21-2077AF488 | |||
| Alexa Fluor 647 | NB21-2077AF647 | |||
| Alexa Fluor 700 | NB21-2077AF700 | |||
| Biotin | NB21-2077B | |||
| DyLight 405 | NB21-2077V | |||
| DyLight 488 | NB21-2077G | |||
| DyLight 550 | NB21-2077R | |||
| DyLight 650 | NB21-2077C | |||
| FITC | NB21-2077F | |||
| HRP | NB21-2077H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2077SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80771 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2000AF405 | |||
| Alexa Fluor 488 | NB21-2000AF488 | |||
| Alexa Fluor 647 | NB21-2000AF647 | |||
| Alexa Fluor 700 | NB21-2000AF700 | |||
| Biotin | NB21-2000B | |||
| DyLight 405 | NB21-2000V | |||
| DyLight 488 | NB21-2000G | |||
| DyLight 550 | NB21-2000R | |||
| DyLight 650 | NB21-2000C | |||
| FITC | NB21-2000F | |||
| HRP | NB21-2000H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2000SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2089AF405 | |||
| Alexa Fluor 488 | NB21-2089AF488 | |||
| Alexa Fluor 647 | NB21-2089AF647 | |||
| Alexa Fluor 700 | NB21-2089AF700 | |||
| Biotin | NB21-2089B | |||
| DyLight 405 | NB21-2089V | |||
| DyLight 488 | NB21-2089G | |||
| DyLight 550 | NB21-2089R | |||
| DyLight 650 | NB21-2089C | |||
| FITC | NB21-2089F | |||
| HRP | NB21-2089H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2089SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2011AF405 | |||
| Alexa Fluor 488 | NB21-2011AF488 | |||
| Alexa Fluor 647 | NB21-2011AF647 | |||
| Alexa Fluor 700 | NB21-2011AF700 | |||
| Biotin | NB21-2011B | |||
| DyLight 405 | NB21-2011V | |||
| DyLight 488 | NB21-2011G | |||
| DyLight 550 | NB21-2011R | |||
| DyLight 650 | NB21-2011C | |||
| FITC | NB21-2011F | |||
| HRP | NB21-2011H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2090SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-2044V | |||
| FITC | NB21-2044F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2044SS | |||
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0010.jpg "Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in HeLa histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
![Histone H4 [ac Lys5] Antibody [NB21-2024]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-NB21-2024-img0011.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0008.jpg "Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody [NB21-2077] - Histone H4 K16ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys16]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2077-img0005.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - WB analysis of H4K16ac in 1) HeLa histone prep lysate and 2) NIH 3T3 histone prep lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Dot blot analysis of H4K16ac in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Dot-Blot-NB21-2077-img0004.jpg)
![Histone H4 [ac Lys16] Antibody [NB21-2077]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-NB21-2077-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Chromatin-Immunoprecipitation-NBP1-30091-img0001.jpg "Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0009.jpg)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Western-Blot-NBP1-30091-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0006.jpg)
![Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Dot-Blot-NBP1-30091-img0002.jpg)
![Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti-Histone H3 Antibody (NB500-171) used at 1:20,000 and Anti-Histone H4 used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12--ac-Lys16--ac-Lys8--ac-Lys5]-Antibody-Western-Blot-NBP2-16848-img0015.jpg "Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti-Histone H3 Antibody (NB500-171) used at 1:20,000 and Anti-Histone H4 used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Histone H4 Antibody [NBP2-16848] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) protein stained by Histone H4K5K8K12K16ac (acetyl Lys5/Lys8/Lys12/Lys16) antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16848-img0014.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-16848] - Human breast cancer, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-16848-img0010.jpg)
![Western Blot: Histone H4 Antibody [NBP2-16848] - WB for total histone in MCF-7 and HCT116 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-16848-img0008.jpg)
![Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-16848-img0012.jpg)
![Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-16848-img0013.jpg)
![Dot Blot: Histone H4 Antibody [NBP2-16848] - Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17) antibody Histone H4 at different dilution as indicated. A: Peptide samples of Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17). B: Peptide samples of Histone H4](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Dot-Blot-NBP2-16848-img0007.jpg)
![Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE NBP2-16848 diluted at 1:10000](http://images.novusbio.com/fullsize/nbp2-16848_rabbit-polyclonal-histone-h4-ac-lys12-ac-lys16-ac-lys8-ac-lys5-antibody-91020242021955.jpg)
![Western Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in HeLa histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[p-Ser1]-Antibody-Western-Blot-NB21-2000-img0004.jpg "Western Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in HeLa histone prep.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in nonmitotic and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red)](http://images.novusbio.com/fullsize/Histone-H4-[p-Ser1]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2000-img0009.jpg)
![Chromatin Immunoprecipitation: Histone H4 [p Ser1] Antibody [NB21-2000] - 2 ug of NB21-2000 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H4-[p-Ser1]-Antibody-Chromatin-Immunoprecipitation-NB21-2000-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [p Ser1] Antibody [NB21-2000] - Histone H4 pS1 antibody was tested in in nonmitotic, prophase, and telophase HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[p-Ser1]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2000-img0008.jpg)
![Dot Blot: Histone H4 [p Ser1] Antibody [NB21-2000] - Analysis of Histone H4 pS1 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[p-Ser1]-Antibody-Dot-Blot-NB21-2000-img0011.jpg)
![Histone H4 [p Ser1] Antibody [NB21-2000]](http://images.novusbio.com/fullsize/Histone-H4-[p-Ser1]-Antibody-NB21-2000-img0012.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0006.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2089-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NB21-2089-img0009.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Detection of H4K20me2 in HeLa histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0003.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of H4K20me2 using peptides stated above in 4, 3, 2 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Dot-Blot-NB21-2089-img0001.jpg)
![Histone H4 [Dimethyl Lys20] Antibody [NB21-2089]](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-NB21-2089-img0010.jpg)
![Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Analysis of Histone H4 R3me1 in NIH/3T3 histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-Western-Blot-NB21-2011-img0002.jpg "Western Blot: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Analysis of Histone H4 R3me1 in NIH/3T3 histone preps.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody [NB21-2011] - Histone H4 R3me1 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2011-img0005.jpg)
![Histone H4 [Monomethyl Arg3] Antibody [NB21-2011]](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Arg3]-Antibody-NB21-2011-img0006.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NB21-2090-img0002.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.")
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NB21-2090-img0001.jpg)
![Simple Western: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Lane view shows a specific band for Histone H4 in 0.5 mg/ml of Hela Nuclear lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Simple-Western-NB21-2090-img0003.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NBP2-42863-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42863-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0006.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-42863-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NBP2-42863-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18 hrs) B. Control with 2.5 ug of pre-immune rabbit IgG C. Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 ug of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunoprecipitation-NBP2-42863-img0007.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NB21-2044-img0006.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NB21-2044] - H4 K8ac antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2044-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NB21-2044] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-2044 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NB21-2044-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - Dot Blot analysis of H4 K8ac using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NB21-2044-img0004.jpg)
![Histone H4 [ac Lys8] Antibody [NB21-2044]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-NB21-2044-img0008.jpg)
![Western Blot: Histone H4 Antibody [NBP2-42856] - Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-42856-img0008.jpg "Western Blot: Histone H4 Antibody [NBP2-42856] - Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Histone H4 Antibody [NBP2-42856] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 protein stained by Histone H4 antibody diluted at 1:5000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:2000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42856-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Rat brain stem. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0004.jpg)
![Western Blot: Histone H4 Antibody [NBP2-42856] - Analysis of various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at a dilution of 1:5000.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-42856-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse fore brain. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse fore brain. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse muscle. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0003.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-42862-img0007.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42862-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of rat fore brain. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse duodenum. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse liver. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse muscle. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0005.jpg)