![Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Western-Blot-NB100-97852-img0006.jpg "Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ch, Pm, Rb, Xp, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Immunogen for A301-844A wthis antibody a synthetic phosphorylated peptide, which represented a portion of human Histone H3 surrounding serine 10 according to the numbering given in entry NP_003520.1 (GeneID 8350). |
| Modification | p Ser10 |
| Predicted Species | Rat (100%), Porcine (100%), Bovine (100%), Rabbit (100%), Zebrafish (100%), Chicken (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H3C1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Flow Cytometry 0.4 ug per 1 million cells in a 150 mcl reaction. Immunofluorescence (ICC) 1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining. Immunohistochemistry 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HIST1H3A Antibody (NB100-97852)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H3C1 |
| Entrez |
|
| Uniprot |
|
![Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-97852-img0004.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.")
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB100-97852-img0003.jpg "Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB")
![Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Flow-Cytometry-NB100-97852-img0002.jpg "Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")