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![Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Western-Blot-NBP2-43535-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43535-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0007.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-43535-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0006.jpg)
![Dot Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of anti-Histone H3 (acetyl Lys18) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys18) antibody 1:5000 dilution. A: Peptide samples of H3K18 un B: Peptide samples of H3K18ac C: Peptide samples of H3K9un D: Peptide samples of H3K9ac E: Peptide samples of H3K14un F: Peptide samples of H3K14ac G: Peptide samples of H3K27un H: Peptide samples of H3K27ac .](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Dot-Blot-NBP2-43535-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunoprecipitation-NBP2-43535-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420122076.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014689.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115031.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242014664.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-910202420115087.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242011459.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects Histone H3K18ac protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Histone H3K18ac stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-91020242063367.jpg)
![Immunocytochemistry/ Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HIST1H3A stained by HIST1H3A antibody (NBP2-43535) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-43535_rabbit-polyclonal-hist1h3a-ac-lys18-antibody-9102024205654.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16842] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16842-img0007.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16842] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0018.jpg)
![Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16842-img0005.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16842-img0013.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16842-img0015.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0004.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - IP Sample: Raji whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 ug of Histone H3 antibody15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16842-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody (NBP2-16842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16842_rabbit-polyclonal-hist1h3a-antibody-9102024202460.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16841] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys9) antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16841-img0004.jpg "Western Blot: HIST1H3A Antibody [NBP2-16841] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys9) antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16841] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16841-img0007.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841] - Paraffin-embedded rat kidney. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16841-img0009.jpg)
![Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16841] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-NBP2-16841-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16841] - Paraffin-embedded mouse brain. Histone H3K9me3 (trimethyl Lys9) antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16841-img0008.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16841] - Immunoprecipitation of Histone H3 (tri-Methyl Lys9) protein from 293T whole cell extracts using 5 ug of Histone H3 (tri-Methyl Lys9). Western blot analysis was performed using Histone H3 (tri-Methyl Lys9). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16841-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HIST1H3A Antibody [NBP2-16841] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K9me3 (Tri-methyl Lys9) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16841_rabbit-polyclonal-hist1h3a-antibody-1610202419414554.jpg)
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Western blot analysis of HIST1H3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0008.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Western blot analysis of HIST1H3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded rat brain tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0005.jpg)
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on Jurkat cell lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0006.jpg)
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on rat liver (1) and mouse lung (2) tissue lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded human kidney tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0004.jpg)
![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0007.jpg "Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis of purified human recombinant histone H3, CenH3, H3.3, H2A, H2B and H4.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (CL4967) [NBP2-61150] - Staining of U-2 OS cells using the Anti-HIST1H3A monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunocytochemistry-Immunofluorescence-NBP2-61150-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human kidney shows strong nuclear positivity in renal tubules cells.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0004.jpg)
![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in human cell line HeLa.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0005.jpg)
![Western Blot: HIST1H3A Antibody (CL4967) [NBP2-61150] - Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Western-Blot-NBP2-61150-img0006.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous tubules.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (CL4967) [NBP2-61150] - Immunohistochemical staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-CL4967-Immunohistochemistry-NBP2-61150-img0003.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16838] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16838-img0010.jpg "Western Blot: HIST1H3A Antibody [NBP2-16838] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - HIST1H3A antibody detects Histone HIST1H3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIST1H3A protein stained by HIST1H3A antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16838-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16838-img0002.jpg)
![Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-16838] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-HIST1H3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16838-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16838] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16838-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16838-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - HIST1H3A Antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIST1H3A protein stained by HIST1H3A Antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16838-img0007.jpg)
![Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of Histone H3 (di-methyl Lys4) antibody The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus. .](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-NBP2-16838-img0006.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 ug of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16838-img0003.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of : A. 30 ug HeLa whole cell lysate/extract (untreated). B. 30 ug HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [921].](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Western-Blot-NBP2-43620-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of : A. 30 ug HeLa whole cell lysate/extract (untreated). B. 30 ug HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [921].")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Analysis of Samples: HCT116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody. Red: Histone H3S10ph (phospho Ser10) protein stained by Histone H3S10ph (phospho Ser10) antibody [921]. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunocytochemistry-Immunofluorescence-NBP2-43620-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Mouse brain. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0006.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Rat brain. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody (921) [NBP2-43620] - Rat brain. Histone H3S10ph (phospho Ser10) antibody [921] diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HIST1H3A-p-Ser10-Antibody-921-Immunohistochemistry-Paraffin-NBP2-43620-img0005.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Western-Blot-NB100-97852-img0006.jpg "Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-97852-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB100-97852-img0003.jpg)
![Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Flow-Cytometry-NB100-97852-img0002.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16837] - Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16837-img0005.jpg "Western Blot: HIST1H3A Antibody [NBP2-16837] - Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16837] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9K14ac (acetyl Lys9/Lys14) protein stained by Histone H3K9K14ac (acetyl Lys9/Lys14) antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16837-img0007.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A Antibody [NBP2-16837] - Histone H3 (acetyl Lys 9, Lys14) antibody detects Histone H3 (acetyl Lys 9, Lys14) protein by western blot analysis, 15% SDS-PAGE.A. 30 ug HeLa whole cell lysate/extract (untreated)B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin treatment for 18 hr)The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16837-img0003.jpg)
![Dot Blot: HIST1H3A Antibody [NBP2-16837] - Dotblot analysis of anti-Histone H3 (acetyl Lys 9, Lys14) antibody with peptide samples. Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys 9, Lys14) antibody at different dilution as indicated. A: Peptide samples of Histone H3.1 B: Peptide samples of Histone H3 (acetyl Lys 9, Lys14)](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Dot-Blot-NBP2-16837-img0006.jpg)
![Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] - Image of HIST1H3A Antibody (9G8) diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26231_mouse-hist1h3a-mab-9g8-27220248573318.jpg "Immunohistochemistry: HIST1H3A Antibody (9G8) [NBP3-26231] - Image of HIST1H3A Antibody (9G8) diluted at 1:300 and staining in paraffin-embedded human salivary gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: HIST1H3A Antibody (9G8) [NBP3-26231] - Positive Western Blot detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate. All lanes: HIST1H3A Antibody at 1: 1000 Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 16 kDa Observed band size: 16 kDa](http://images.novusbio.com/fullsize/nbp3-26231_mouse-hist1h3a-mab-9g8-28220241140916.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (9G8) [NBP3-26231] - Staining of HepG2 cell with HIST1H3A Antibody (9G8) at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26231_mouse-hist1h3a-mab-9g8-28220241247511.jpg)
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-2622024151064.jpg "Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.")
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-272202485630.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Staining of A549 with HIST1H3A [ac Lys18] Antibody (30A7) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 490-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202412493638.jpg)
![Flow Cytometry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Overlay Peak curve showing A549 cells stained with HIST1H3A [ac Lys18] Antibody (30A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202414312785.jpg)
![Immunohistochemistry: Histone H3T Antibody (CL4968) [NBP2-61643] - Staining of human cerebral cortex shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/Histone-H3T-Antibody-CL4968-Immunohistochemistry-NBP2-61643-img0004.jpg "Immunohistochemistry: Histone H3T Antibody (CL4968) [NBP2-61643] - Staining of human cerebral cortex shows moderate to strong nuclear immunoreactivity in neuronal and glial cells.")
![Immunohistochemistry: Histone H3T Antibody (CL4968) [NBP2-61643] - Staining of human prostate shows moderate nuclear positivity in glandular and smooth muscle cells.](http://images.novusbio.com/fullsize/Histone-H3T-Antibody-CL4968-Immunohistochemistry-NBP2-61643-img0001.jpg)
![Immunohistochemistry: Histone H3T Antibody (CL4968) [NBP2-61643] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells as well as in cells in lamina propria.](http://images.novusbio.com/fullsize/Histone-H3T-Antibody-CL4968-Immunohistochemistry-NBP2-61643-img0002.jpg)
![Immunohistochemistry: Histone H3T Antibody (CL4968) [NBP2-61643] - Staining of human fallopian tube shows strong nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/Histone-H3T-Antibody-CL4968-Immunohistochemistry-NBP2-61643-img0003.jpg)