![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Western blot analysis of HIST1H3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0008.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Western blot analysis of HIST1H3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: rat liver tissue lysate Lane 3: mouse lung tissue lysate")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ChIP, ICC/IF, IHC, IP |
| Clone | JU43-26 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human HIST1H3A aa 1-50 / 136 (acetyl K14) . (SwissProt: P68431 Human; SwissProt: P68433 Mouse; SwissProt: Q6LED0 Rat) |
| Localization | Nucleus. Chromosome. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | H3C1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | H3C1 |
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0003.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded rat brain tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0005.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded rat brain tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.")
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on Jurkat cell lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0006.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on Jurkat cell lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.")
![Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on rat liver (1) and mouse lung (2) tissue lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Western-Blot-NBP2-75522-img0007.jpg "Western Blot: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of Histone H3 (acetyl K14) on rat liver (1) and mouse lung (2) tissue lysate using anti-Histone H3 (acetyl K14) antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0001.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunocytochemistry-Immunofluorescence-NBP2-75522-img0002.jpg "Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Staining Histone H3 (acetyl K14) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded human kidney tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-JU43-26-Immunohistochemistry-Paraffin-NBP2-75522-img0004.jpg "Immunohistochemistry-Paraffin: HIST1H3A Antibody (JU43-26) [NBP2-75522] - Analysis of paraffin-embedded human kidney tissue using anti-Histone H3 (acetyl K14) antibody. Counter stained with hematoxylin.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")