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HCN4 Antibody (SHG 1E5)

Images

 
Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439] - Analysis of 25 ug of rat eye lysate onto an SDS polyacrylamide gel.
Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.
Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, ICC/IF
Clone
SHG 1E5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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HCN4 Antibody (SHG 1E5) Summary

Immunogen
Synthetic peptide sequence corresponding to residues S H G S L L L P P A S S P P P P Q V P Q R R G T P P L T P G R L T Q D L K L of HCN4.
Specificity
HCN4 (SHG 1E5)
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
HCN4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:1000
  • Immunohistochemistry-Paraffin 1:10 - 1:100
  • Western Blot 1:5000
Application Notes
WB: Detects an approx.132 KDa protein representing HCN4.
Publications
Read Publications using NB100-74439.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for HCN4 Antibody (SHG 1E5)

  • HCN4
  • hyperpolarization activated cyclic nucleotide-gated cation channel 4
  • hyperpolarization activated cyclic nucleotide-gated potassium channel 4
  • potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
  • SSS2

Background

HCN3 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. HCN3 has been detected in the brain, but with less expression than other HCN channels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HCN4 Antibody (NB100-74439)(2)


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HCN4 Antibody (NB100-74439). (Showing 1 - 2 of 2 FAQs).

  1. I was looking for HCN4 antiboby that attaches to the extra cellular domain of the channel tested on mouse and human for immunio stoaining..
    • The immunogen for our monoclonal antibody to HCN4, is actually the rat protein. The sequence comes from amino acids 1046 - 1083 of the 1198 amino acid protein, but unfortunately, it is from a cytoplasmic domain. This is the only HCN4 Ab that we have tested for IHC and that has been validated for use with mouse and human.
  2. I need an anti human HCN4 antibody for sorting. I was wondering if your product HCN4 antibody (NB110-74735) can react with the extracellular portions of HCN4 and is applicable for FACS?
    • NB110-74735 was raised against a synthetic peptide corresponding to a region that covered both cytoplasmic and extracellular regions of the HCN4 protein. We don't routinely map our antibodies, and we don't know where specifically this antibody binds its target. NB100-74439 may be a better option for you, as the immunogen sequence is provided on our website, but we have not validated it for Flow/FACS.

Secondary Antibodies

 

Isotype Controls

Additional HCN4 Products

Array NB100-74439

Research Areas for HCN4 Antibody (NB100-74439)

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Blogs on HCN4.

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Bioinformatics

Gene Symbol HCN4
Entrez
Uniprot