![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0010.jpg "Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, MA |
| Clone | S71-37 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein amino acids 761-863 of rat HCN2 |
| Localization | Membrane |
| Specificity | Detects approx 95kDa. No cross-reactivity against HCN1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | HCN2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of HCN2 Antibody was sufficient for detection of HCN2 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | HCN2 |
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0013.jpg "Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN2 Antibody (D) Composite.")
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0012.jpg "Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.")
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0009.jpg "Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Human hippocampal tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0006.jpg "Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Human hippocampal tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.")
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (NBP2-12895). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) HCN2 Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0014.jpg "Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (NBP2-12895). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) HCN2 Antibody. (D) Hoechst (E) Composite.")
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0011.jpg "Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")