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HAND1 Antibody (OTI1G10)

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Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; ...read more
Immunocytochemistry/ Immunofluorescence: HAND1 Antibody (OTI1G10) [NBP2-00576] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.
Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Jurkat cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: HAND1 Antibody (OTI1G10) [NBP2-00576] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Flow Cytometry: HAND1 Antibody (OTI1G10) [NBP2-00576] - Analysis of Hela cells, using anti-HAND1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Func, ICC/IF
Clone
OTI1G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.82 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71459AF350
Alexa Fluor 405
NBP2-71459AF405
Alexa Fluor 488
NBP2-71459AF488
Alexa Fluor 532
NBP2-71459AF532
Alexa Fluor 594
NBP2-71459AF594
Alexa Fluor 647
NBP2-71459AF647
Alexa Fluor 700
NBP2-71459AF700
Alexa Fluor 750
NBP2-71459AF750
Allophycocyanin
NBP2-71459APC
Allophycocyanin/Cy7
NBP2-71459APCCY7
Biotin
NBP2-71459B
CoraFluor 1
NBP2-71459CL1
DyLight 350
NBP2-71459UV
DyLight 405
NBP2-71459V
DyLight 488
NBP2-71459G
DyLight 550
NBP2-71459R
DyLight 594
NBP2-71459DL594
DyLight 650
NBP2-71459C
DyLight 680
NBP2-71459FR
DyLight 755
NBP2-71459IR
FITC
NBP2-71459F
HRP
NBP2-71459H
Janelia Fluor 525
NBP2-71459JF525
Janelia Fluor 549
NBP2-71459JF549
Janelia Fluor 585
NBP2-71459JF585
Janelia Fluor 635
NBP2-71459JF635
Janelia Fluor 646
NBP2-71459JF646
Janelia Fluor 669
NBP2-71459JF669
PE
NBP2-71459PE
PE/Atto594
NBP2-71459PEATT594
PE/Cy5.5
NBP2-71459PECY55
PE/Cy7
NBP2-71459PECY7
PerCP
NBP2-71459PCP
mFluor Violet 450 SE
NBP2-71459MFV450
mFluor Violet 500 SE
NBP2-71459MFV500
mFluor Violet 610 SE
NBP2-71459MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71459
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HAND1 Antibody (OTI1G10) Summary

Immunogen
Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HAND1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • In vitro assay Reported in scientific literature (PMID:33316598)
  • Western Blot 1:500
Theoretical MW
23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-00576 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HAND1 Antibody (OTI1G10)

  • BHLHA27
  • bHLHa27heart- and neural crest derivatives-expressed protein 1
  • Class A basic helix-loop-helix protein 27
  • eHand
  • Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1
  • HAND1
  • heart and neural crest derivatives expressed 1
  • Hxt
  • Thing1

Background

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HAND1 Antibody
(OTI1G10)
NBP2-00576
HAND1 Antibody (OTI1G10)
Species: Hu, Mu, Rt
Applications: WB, Flow, Func, ICC/IF

Publications for HAND1 Antibody (NBP2-00576)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: In vitro.


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Bioinformatics

Gene Symbol HAND1
Entrez