![Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425483.jpg "Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1562 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GRK2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for GRK2 Antibody (NBP3-25491)Find related products by research area.
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"Freeze!" - Arrestin Antibodies Used in New Serotonin Syndrome Study The beta-arrestin family regulate receptor binding of G-proteins, a group of seven transmembrane receptor proteins which includes the adrenergic, dopamine and serotonin receptors. Recently, arrestin antibodies were used in a study into Serotonin Syndr... Read full blog post. |
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| Gene Symbol | GRK2 |
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-212024939175.jpg "Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249432853.jpg "Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425466.jpg "Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425437.jpg "Immunocytochemistry/Immunofluorescence: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-212024940814.jpg "Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")