![Western Blot: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GSC monoclonal antibody.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Western-Blot-NBP2-72409-img0001.jpg "Western Blot: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GSC monoclonal antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready |
| Clone | OTI1D7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 107-257 of human GSC (NP_776248) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GSC |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GSC |
![Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human prostate tissue using anti-GSC mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72409-img0007.jpg "Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human prostate tissue using anti-GSC mouse monoclonal antibody.")
![Flow Cytometry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GSC antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72409-img0003.jpg "Flow Cytometry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-GSC antibody, and then analyzed by flow cytometry.")
![Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GSC mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72409-img0004.jpg "Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GSC mouse monoclonal antibody.")
![Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human liver tissue using anti-GSC mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72409-img0005.jpg "Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human liver tissue using anti-GSC mouse monoclonal antibody.")
![Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human pancreas tissue using anti-GSC mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72409-img0006.jpg "Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human pancreas tissue using anti-GSC mouse monoclonal antibody.")
![Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human Kidney tissue using anti-GSC mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72409-img0008.jpg "Immunohistochemistry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - Staining of paraffin-embedded Human Kidney tissue using anti-GSC mouse monoclonal antibody.")
![Flow Cytometry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSC.](http://images.novusbio.com/fullsize/Goosecoid-Antibody-OTI1D7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72409-img0002.jpg "Flow Cytometry: Goosecoid Antibody (OTI1D7) - Azide and BSA Free [NBP2-72409] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSC.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")