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Glutaminase Activity Assay Kit (Colorimetric)

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Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] - Standard Reference Curve
Glutaminase Activity Assay Kit (Colorimetric) [NBP3-24496] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma, animal tissue and plant tissue
Standard Curve Range
0.003 - 18.0 U/L
Sensitivity
0.003 U/L

Order Details

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Glutaminase Activity Assay Kit (Colorimetric) Summary

Description
Detection instrument: Microplate reader (440 - 460 nm, optimum wavelength: 450 nm)
Assay time: 80 min
Standard Curve Range
0.003 - 18.0 U/L
Sensitivity
0.003 U/L
Inter-Assay
%CV < 8.3
Intra-Assay
%CV < 5
Kit Type
Activity Assay Kit (Colorimetric)
Gene
GLS

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Microplate
  2. Plate Sealer
  3. Reagent 1: Substrate A
  4. Reagent 2: Standard
  5. Reagent 3: Diluent
  6. Reagent 4: Enzyme Reagent
  7. Reagent 5: Buffer Solution
  8. Reagent 6: Substrate B
  9. Reagent 7: Accelerator
  10. Reagent 8: Chromogenic Agent

Alternate Names for Glutaminase Activity Assay Kit (Colorimetric)

  • AAD20
  • EC 3.5.1.2
  • GLS
  • GLS1DKFZp686O15119
  • glutaminase C
  • glutaminase kidney isoform, mitochondrial
  • Glutaminase
  • glutaminase, phosphate-activated
  • K-Glutaminase
  • KIAA0838FLJ10358
  • L-Glutamine Amidohydrolase

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GLS