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GLP1 Antibody (10) [CoraFluor™ 1]

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Product Details

Summary
Reactivity MaSpecies Glossary
Applications WB, ELISA
Clone
10
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-05180AF350
Alexa Fluor 405
NBP1-05180AF405
Alexa Fluor 488
NBP1-05180AF488
Alexa Fluor 532
NBP1-05180AF532
Alexa Fluor 594
NBP1-05180AF594
Alexa Fluor 647
NBP1-05180AF647
Alexa Fluor 700
NBP1-05180AF700
Alexa Fluor 750
NBP1-05180AF750
DyLight 350
NBP1-05180UV
DyLight 405
NBP1-05180V
DyLight 488
NBP1-05180G
DyLight 550
NBP1-05180R
DyLight 594
NBP1-05180DL594
DyLight 650
NBP1-05180C
DyLight 680
NBP1-05180FR
DyLight 755
NBP1-05180IR
FITC
NBP1-05180F
HRP
NBP1-05180H
Janelia Fluor 525
NBP1-05180JF525
Janelia Fluor 549
NBP1-05180JF549
Janelia Fluor 585
NBP1-05180JF585
Janelia Fluor 635
NBP1-05180JF635
Janelia Fluor 646
NBP1-05180JF646
Janelia Fluor 669
NBP1-05180JF669
Unconjugated
NBP1-05180
mFluor Violet 450 SE
NBP1-05180MFV450
mFluor Violet 500 SE
NBP1-05180MFV500
mFluor Violet 610 SE
NBP1-05180MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-05180
BSA Free
NBP1-05180
BSA Free
NBP1-05180-0.025mg
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GLP1 Antibody (10) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Synthetic GLP-1(7-17)
Epitope
Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.
Specificity
Binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. Cross-reacts with Liraglutide.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GCG
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for GLP1 Antibody (10) [CoraFluor™ 1]

  • glicentin-related polypeptide
  • GLP1
  • GLP-1
  • glucagon-like peptide 1
  • GRPP

Background

Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from enteroendocrine L cells in response to ingested nutrients. The closely related peptides glucagon-like peptide (GLP-1) and glucagon have opposing effects on blood glucose. GLP-1 induces glucose-dependent insulin secretion in the pancreas, while glucagon stimulates gluconeogenesis and glycogenolysis in the liver. Glucagon is processed from a large precursor, proglucagon, in a tissue-specific manner in pancreatic alpha-cells. The identification of a hybrid peptide acting as both a GLP-1 agonist and a glucagon antagonist would provide a novel approach for the treatment of type 2 diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GCG