![Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/GLI-2-Antibody-Western-Blot-NB600-874-img0012.jpg "Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This affinity-purified antibody is directed against human Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with GLI-2 from human and chimpanzee based on the immunizing sequence. Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070) |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GLI2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 27760825). |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 02/28/2021 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for GLI-2 Antibody (NB600-874)Find related products by research area.
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![Immunohistochemistry-Paraffin: GLI-2 Antibody [NB600-874] - Mouse adenocarcinoma section shows high and diffuse nuclear Gli2 expression. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NB600-874-img0013.jpg "Immunohistochemistry-Paraffin: GLI-2 Antibody [NB600-874] - Mouse adenocarcinoma section shows high and diffuse nuclear Gli2 expression. IHC-P image submitted by a verified customer review.")
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