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GDF-15 Antibody (CL0328)

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Orthogonal Strategies: Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining in human placenta and skeletal muscle tissues using NBP2-52917 antibody. Corresponding GDF15 RNA-seq data are ...read more
Genetic Strategies: Western Blot: GDF-15 Antibody (CL0328) [NBP2-52917] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GDF15 antibody. Remaining ...read more
Immunocytochemistry/ Immunofluorescence: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining of A549 cells using the Anti-GDF15 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and ...read more
Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining of human prostate shows strong granular cytoplasmic positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining of human rectum shows strong cytoplasmic positivity in enteroendocrine cells.
Immunohistochemistry-Paraffin: GDF-15 Antibody (CL0328) [NBP2-52917] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clone
CL0328
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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GDF-15 Antibody (CL0328) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQL
Epitope
NQSWEDSNTD
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GDF15
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
GDF-15 Recombinant Protein Antigen (NBP2-52917PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for GDF-15 Antibody (CL0328)

  • GDF15
  • GDF-15
  • growth differentiation factor 15
  • growth/differentiation factor 15
  • Macrophage inhibitory cytokine 1
  • MIC-1
  • MIC-1NSAID-activated gene 1 protein
  • MIC1Prostate differentiation factor
  • NAG-1
  • NAG-1NSAID-regulated gene 1 protein
  • NSAID (nonsteroidal inflammatory drug)-activated protein 1
  • PDF
  • PDFGDF-15
  • PLAB
  • PLABNRG-1
  • Placental bone morphogenetic protein
  • Placental TGF-beta
  • PTGF-beta
  • PTGFBPTGF-beta

Background

Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.

GDF15 antibodies are useful for studies on inflamation and immune system research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GDF-15 Antibody (NBP2-52917) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on GDF-15.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

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Bioinformatics

Gene Symbol GDF15