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GBP6 Antibody

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Orthogonal Strategies: Western Blot: GBP6 Antibody [NBP1-84117] - Analysis in human cell lines A-431 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GBP6 Antibody [NBP1-84117] - Analysis in human esophagus and colon tissues. Corresponding GBP6 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GBP6 Antibody [NBP1-84117] - Staining of human esophagus shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: GBP6 Antibody [NBP1-84117] - Staining of human oral mucosa shows weak cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: GBP6 Antibody [NBP1-84117] - Staining of human cervix, uterine shows weak cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: GBP6 Antibody [NBP1-84117] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: GBP6 Antibody [NBP1-84117] - Staining of human tonsil shows moderate cytoplasmic positivity in squamous epithelial cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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GBP6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EREHLLREQIMMLEHTQKVQNDWLHEGFKKKYEEMNAEISQFKRMIDTTKNDDTPWIARTLDNLADELTAILSAPAKLIGHGVKGV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GBP6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GBP6 Protein (NBP1-84117PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GBP6 Antibody

  • DKFZp686G0786
  • FLJ39135
  • GBP-6
  • GTP-binding protein 6
  • Guanine nucleotide-binding protein 6
  • guanylate binding protein family, member 6
  • guanylate-binding protein 6

Background

Guanylate-binding proteins, such as GBP6, are induced by interferon and hydrolyze GTP to both GDP and GMP (Olszewski et al., 2006 [PubMed 16689661]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GBP6