Recombinant Human Galectin-1 Protein Summary
Description |
A single non-glycosylated polypeptide chain containing 134 amino acids corresponding to Galectin-1 Source: E. coli Uniprot ID: P09382 Amino Acid Sequence: ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions |
Details of Functionality |
Galectin 1 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human blood monocytes is in a concentration range of 1.0-10 ng/ml. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
LGALS1 |
Purity |
>95%, by SDS-PAGE and HPLC |
Endotoxin Note |
Less than 0.1 EU/ug of Galectin-1 as determined by LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
14.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Publications |
|
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a 0.2 um filtered solution in PBS pH 7.0, 3mM beta-Me, 0.02% Tween 20. |
Preservative |
5% Trehalose |
Concentration |
LYOPH |
Purity |
>95%, by SDS-PAGE and HPLC |
Reconstitution Instructions |
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Alternate Names for Recombinant Human Galectin-1 Protein
Background
Human Galectin-1 also named BHL, Galaptin, GBP, L-14 and LGALS1, is belonging to the galectins family and it is encoded by the LGALS1 gene in human. Galectin-1 is expressed by the endometrial stromal cells throughout the menstrual cycle. Galectin-1 contains a single carbohydrate recognition domain through which it can bind glycans both as a monomer and as a homodimer. Dimers are non-covenantly bound and will spontaneously disassociate in low concentration. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 is thought to play a role in the immunosuppression required for a successful pregnancy. Human Galectin-1 shares 88 %-90 % amino acid sequence identity with rat and mouse.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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