Home » Galectin-1 » Galectin-1 Lysates » Galectin-1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

Galectin-1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Galectin 1 Overexpression Lysate (Adult Normal) [NBL1-12496] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Galectin 1.
Western Blot: Galectin 1 Overexpression Lysate (Adult Normal) [NBL1-12496] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Galectin 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Galectin-1 Overexpression Lysate Summary

Description

Galectin-1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204674

Accession#: NM_002305

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
LGALS1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Galectin-1 Overexpression Lysate

  • 14 kDa laminin-binding protein
  • 14 kDa lectin
  • Beta-galactoside-binding lectin L-14-I
  • BHL
  • DKFZp686E23103
  • GAL1
  • gal-1
  • Galaptin
  • galectin 1
  • Galectin1
  • Galectin-1
  • GBP
  • HBL
  • HLBP14
  • HPL
  • L-14
  • Lactose-binding lectin 1
  • Lectin galactoside-binding soluble 1
  • lectin, galactoside-binding, soluble, 1
  • LGALS1
  • Putative MAPK-activating protein PM12
  • S-Lac lectin 1

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Galectin-3
Antibody [Unconjugate
AF1197
Western Blot Galectin-3 Antibody [Unconjugated]
Immunohistochemistry Galectin-3 Antibody [Unconjugated]
Simple Western Galectin-3 Antibody [Unconjugated]
(2 Reviews)
(42 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
Placental
Lactogen/CSH1 Antibod ...
NBP2-46376
Immunohistochemistry Placental Lactogen/CSH1 Antibody (OTI2G4)
Immunohistochemistry Placental Lactogen/CSH1 Antibody (OTI2G4)
Immunohistochemistry Placental Lactogen/CSH1 Antibody (OTI2G4)
Species: Hu
Applications: IHC, IHC-P, WB
CSH2 Antibody
NBP2-16019
Western Blot CSH2 Antibody
Immunohistochemistry-Paraffin CSH2 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Galectin-4
Antibody [Unconjugate
AF2128
Immunohistochemistry Galectin-4 Antibody [Unconjugated]
(5 Publications)
Species: Mu
Applications: IHC, WB
Galectin-7
Antibody [Unconjugate
AF1339
Western Blot Galectin-7 Antibody [Unconjugated]
Simple Western Galectin-7 Antibody [Unconjugated]
(5 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Simple Western, WB
GALK1 Antibody
NBP1-81853
Western Blot GALK1 Antibody
Immunocytochemistry/ Immunofluorescence GALK1 Antibody
Western Blot GALK1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
IL-10 [Biotin]
DY417
N/A IL-10 [Biotin]
(302 Publications)
Species: Mu
Applications: ELISA
CD4 Antibody - BSA
Free
NBP1-19371
Immunohistochemistry-Frozen CD4 Antibody - BSA Free
Immunohistochemistry-Paraffin CD4 Antibody - BSA Free
Flow Cytometry CD4 Antibody - BSA Free
(9 Reviews)
(50 Publications)
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Galectin-10
Antibody [Unconjugate
AF5447
Western Blot Galectin-10 Antibody [Unconjugated]
Intracellular Staining by Flow Cytometry Galectin-10 Antibody [Unconjugated]
Intracellular Staining by Flow Cytometry Galectin-10 Antibody [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, ICFlow, WB
CD45 Antibody
[Unconjugated]
AF114
Immunohistochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry CD45 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence CD45 Antibody [Unconjugated]
(1 Review)
(70 Publications)
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
Galectin-8
Antibody [Unconjugate
AF1305
Western Blot Galectin-8 Antibody [Unconjugated]
Simple Western Galectin-8 Antibody [Unconjugated]
Immunohistochemistry Galectin-8 Antibody [Unconjugated]
(1 Review)
(30 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Galectin-2 Antibody
AF6667
Western Blot Galectin-2 Antibody
Immunohistochemistry Galectin-2 Antibody
Species: Mu
Applications: IHC, WB
Galanin Antibody
NBP2-33582
Immunocytochemistry/ Immunofluorescence Galanin Antibody
Immunohistochemistry-Paraffin Galanin Antibody
Immunohistochemistry-Paraffin Galanin Antibody
(1 Review)
(2 Publications)
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
GCET2 Antibody
NBP1-82693
Western Blot GCET2 Antibody
Immunohistochemistry-Paraffin GCET2 Antibody
(1 Review)
Species: Hu
Applications: IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Fibronectin
Antibody - BSA Free
NBP1-91258
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
Western Blot Fibronectin Antibody - BSA Free
(11 Reviews)
(56 Publications)
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
CD8 Antibody
(53-6.7) - BSA Free
NBP1-49045
Immunohistochemistry-Paraffin CD8 Antibody (53-6.7) - BSA Free
Immunocytochemistry/ Immunofluorescence CD8 Antibody (53-6.7) - BSA Free
Immunohistochemistry-Frozen CD8 Antibody (53-6.7) - BSA Free
(3 Reviews)
(28 Publications)
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc

beta-Galactosidase-1/G ...
NBP2-45731
Western Blot beta-Galactosidase-1/GLB1 Antibody (OTI1C9)
Immunohistochemistry beta-Galactosidase-1/GLB1 Antibody (OTI1C9)
Immunohistochemistry beta-Galactosidase-1/GLB1 Antibody (OTI1C9)
(6 Publications)
Species: Ca, Hu, Pm
Applications: IHC, IHC-P, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Galectin-1
Overexpression Lysate
NBL1-12496
Galectin-1 Overexpression Lysate
Species: Hu
Applications: WB

Publications for Galectin-1 Lysate (NBL1-12496) (0)

There are no publications for Galectin-1 Lysate (NBL1-12496).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Galectin-1 Lysate (NBL1-12496) (0)

There are no reviews for Galectin-1 Lysate (NBL1-12496). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Galectin-1 Lysate (NBL1-12496) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Galectin-1 Products

Research Areas for Galectin-1 Lysate (NBL1-12496)

Find related products by research area.

Blogs on Galectin-1

There are no specific blogs for Galectin-1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Galectin-1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol LGALS1