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GADD153/CHOP Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-98742AF350
Alexa Fluor 405
NBP2-98742AF405
Alexa Fluor 488
NBP2-98742AF488
Alexa Fluor 532
NBP2-98742AF532
Alexa Fluor 594
NBP2-98742AF594
Alexa Fluor 647
NBP2-98742AF647
Alexa Fluor 700
NBP2-98742AF700
Alexa Fluor 750
NBP2-98742AF750
Allophycocyanin
NBP2-98742APC
Biotin
NBP2-98742B
DyLight 350
NBP2-98742UV
DyLight 405
NBP2-98742V
DyLight 488
NBP2-98742G
DyLight 550
NBP2-98742R
DyLight 594
NBP2-98742DL594
DyLight 650
NBP2-98742C
DyLight 680
NBP2-98742FR
DyLight 755
NBP2-98742IR
FITC
NBP2-98742F
HRP
NBP2-98742H
Janelia Fluor 525
NBP2-98742JF525
Janelia Fluor 549
NBP2-98742JF549
Janelia Fluor 646
NBP2-98742JF646
Janelia Fluor 669
NBP2-98742JF669
PE
NBP2-98742PE
PerCP
NBP2-98742PCP
Unconjugated
NBP2-98742-100ul
mFluor Violet 450 SE
NBP2-98742MFV450
mFluor Violet 500 SE
NBP2-98742MFV500
mFluor Violet 610 SE
NBP2-98742MFV610
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GADD153/CHOP Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human GADD153/CHOP.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDIT3
Purity
Antigen and protein A Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for GADD153/CHOP Antibody [CoraFluor™ 1]

  • C/EBP-homologous protein 10
  • C/EBP-homologous protein
  • CCAAT/enhancer-binding protein homologous protein
  • CEBP zeta
  • CHOP
  • CHOP10 CEBPZ
  • CHOP10
  • CHOP-10
  • CHOPC/EBP zeta
  • DDIT3
  • DDIT-3
  • DNA damage-inducible transcript 3 protein
  • DNA-damage-inducible transcript 3
  • GADD153
  • Growth arrest and DNA damage-inducible protein GADD153

Background

GADD153 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol DDIT3