![Western Blot: G9a/EHMT2 Antibody [NBP3-18664] - Figure 2. Western blot analysis using the antibody directed against G9a/EHMT2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a/EHMT2 , diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.](http://images.novusbio.com/fullsize/G9a-EHMT2-Antibody-Western-Blot-NBP3-18664-img0001.jpg "Western Blot: G9a/EHMT2 Antibody [NBP3-18664] - Figure 2. Western blot analysis using the antibody directed against G9a/EHMT2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a/EHMT2 , diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against mouse G9a/EHMT2 (Protein G9a/EHMT2), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein (1). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EHMT2 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: G9a/EHMT2 Antibody [NBP3-18664] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a/EHMT2 . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.](http://images.novusbio.com/fullsize/G9a-EHMT2 Antibody-ELISA-NBP3-18664-img0002.jpg "ELISA: G9a/EHMT2 Antibody [NBP3-18664] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a/EHMT2 . The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
