Immunoblotting reported in scientific literature (PMID 22019092)
Immunohistochemistry reported in scientific literature (PMID 28205605)
Western Blot 0.5 ug/ml
Application Notes
In Western blot analysis, a band is seen at ~60 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Chicken reactivity reported in scientific literature (PMID: 22019092).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for FTO Antibody
Alpha-ketoglutarate-dependent dioxygenase FTO
EC 1.14.11.-
fat mass and obesity associated
Fat mass and obesity-associated protein
FTO
KIAA1752protein fto
MGC5149
Background
FTO (alpha-ketoglutarate-dependent dioxygenase FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine, low activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and is inactive towards 1-methylguanine or double-stranded DNA. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succinate etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Zuidhof H, Müller C, Kortman G et al. Oncogenic functions of the m6A demethylase FTO in breast cancer cells involving translational upregulation of C/EBP?-LIP bioRxiv 2023-09-23 (WB, Human)
FAQs for FTO Antibody (NB110-60935). (Showing 1 - 1 of 1 FAQs).
Do you have any antibodies against FTO, where you can indicate the sequence of the immunogen?
With regards to other FTO antibodies that we do list the peptide sequence for, the immunogen sequence for catalog number NB110-60935 can be found in the range of amino acids 400-505. Many of the exact immunogen sequences used for our products are proprietary and cannot be disclosed. In these instances we try to provide a reasonable amino acid range to help in your decision.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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