![Western Blot: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Western-Blot-NBP2-70775-img0001.jpg "Western Blot: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FRK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FRK.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI6D2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human FRK (NP_002022) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FRK |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 58.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Frk Antibody (NBP2-70775)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FRK |
![Immunocytochemistry/Immunofluorescence: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70775-img0003.jpg "Immunocytochemistry/Immunofluorescence: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FRK.")
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Ovary tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0009.jpg "Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Ovary tissue using anti-FRK mouse monoclonal antibody.")
![Flow Cytometry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells transfected with either Frk Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FRK antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70775-img0002.jpg "Flow Cytometry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - HEK293T cells transfected with either Frk Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FRK antibody , and then analyzed by flow cytometry.")
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0004.jpg "Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FRK mouse monoclonal antibody.")
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0005.jpg "Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FRK mouse monoclonal antibody.")
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human colon tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0006.jpg "Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human colon tissue using anti-FRK mouse monoclonal antibody.")
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human endometrium tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0007.jpg "Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human endometrium tissue using anti-FRK mouse monoclonal antibody.")
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Kidney tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0008.jpg "Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human Kidney tissue using anti-FRK mouse monoclonal antibody.")
![Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human pancreas tissue using anti-FRK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Frk-Antibody-OTI6D2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70775-img0010.jpg "Immunohistochemistry: Frk Antibody (OTI6D2) - Azide and BSA Free [NBP2-70775] - Staining of paraffin-embedded Human pancreas tissue using anti-FRK mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")