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Frizzled-6 Antibody [Unconjugated]

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Frizzled-6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Frizzled-6 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Frizzled-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3149) at 10 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3149-SP
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Frizzled-6 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Frizzled-6
His19-Val153
Accession # O60353
Specificity
Detects human Frizzled-6 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD6
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3149 in the following applications:
Publications
Read Publication using
AF3149 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-6 Antibody [Unconjugated]

  • frizzled (Drosophila) homolog 6
  • frizzled homolog 6 (Drosophila)
  • Frizzled6
  • Frizzled-6
  • FZ6
  • fz-6
  • FZD6
  • Hfz6
  • seven transmembrane helix receptor

Background

Frizzled-6 is a member of the Frizzled/Smoothened family of G-protein coupled receptors that mediate the Wnt beta-Catenin signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Frizzled-6 Antibody [Unconjugated]
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready

Publications for Frizzled-6 Antibody (AF3149)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(1)
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Human
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Review for Frizzled-6 Antibody (AF3149) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF3149:
Filter by Applications
Flow
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
 Flow Human 01/07/2015
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Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 24274766
SpeciesHuman

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Secondary Antibodies

 

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Recent Reviews

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 Verified Customer
01/07/2015
Application: Flow
Species: Human
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Bioinformatics

Gene Symbol FZD6
Uniprot