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Frataxin Antibody (FXN/2124) - Azide and BSA Free

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Western Blot: Frataxin Antibody (FXN/2124) - Azide and BSA Free [NBP2-79936] - Analysis of Human Panc-2 and HepG2 cell lysate using Frataxin Mouse Monoclonal Antibody (FXN/2124).
Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) - Azide and BSA Free [NBP2-79936] - Formalin-fixed, paraffin-embedded human Pancreas stained with Frataxin Mouse Monoclonal Antibody (FXN/2124).
Immunohistochemistry-Paraffin: Frataxin Antibody (FXN/2124) - Azide and BSA Free [NBP2-79936] - Formalin-fixed, paraffin-embedded human Pancreas stained with Frataxin Mouse Monoclonal Antibody (FXN/2124).
Protein Array: Frataxin Antibody (FXN/2124) - Azide and BSA Free [NBP2-79936] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Frataxin antibody (FXN/2124).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, MA
Clone
FXN/2124
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Frataxin Antibody (FXN/2124) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79802).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Recombinant fragment of human Frataxin (FXN) protein (around aa 57-210) (exact sequence is proprietary) (Uniprot: Q16595)
Localization
Cytoplasmic (Mitochondria)
Marker
Marker of Friedreich Ataxia
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FXN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1 - 2 ug/mL
  • Immunohistochemistry-Paraffin 1 - 2 ug/mL
  • Protein Array
  • Western Blot 1 - 2 ug/mL
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Frataxin Antibody (FXN/2124) - Azide and BSA Free

  • CyaY
  • EC 1.16.3.1
  • FA
  • FARRmitochondrial
  • frataxin
  • Friedreich ataxia protein
  • Friedreich ataxia
  • Fxn
  • X25MGC57199

Background

Frataxin encodes a mitochondrial protein which belongs to FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FXN