![Western Blot: FOXG1 Antibody [NB100-1099] - (1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1099_goat-polyclonal-foxg1-antibody-16520239183341.jpg "Western Blot: FOXG1 Antibody [NB100-1099] - (1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-HQNQGSSSNPLIH corresponding to C-Terminus according to NP_005240.3. |
| Predicted Species | Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | FOXG1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 48kDa band observed in lysates of cell line U251, and approx. 48-50kDa in Human Cerebellum and Cerebral Cortex lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B), and 50.5kDa according to CAA55038.1 (FOXG1A). An additional band at 70-75kDa was observed in some lysates and was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. |
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| Control |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for FOXG1 Antibody (NB100-1099)Find related products by research area.
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![Western Blot: FOXG1 Antibody [NB100-1099] - (0.5ug/ml) staining of Human Cerebellum (A) and Cerebral Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1099_goat-polyclonal-foxg1-antibody-16520239183351.jpg "Western Blot: FOXG1 Antibody [NB100-1099] - (0.5ug/ml) staining of Human Cerebellum (A) and Cerebral Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: FOXG1 Antibody [NB100-1099] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-1099_goat-polyclonal-foxg1-antibody-1652023922511.jpg "Flow Cytometry: FOXG1 Antibody [NB100-1099] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
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