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Follistatin-like 1/FSTL1 Antibody [Unconjugated]

 (20 Publications)
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Proliferation defects in Fstl1-/- ureteric epithelial cells.(A-D) Analysis of cell proliferation on transverse sections of wild-type (A, C) and Fstl1-/- (B, D) ureters by BrdU incorporation at E15.5 (A, B) and E16.5 (distal segment) (C, D). Arrows point to representative proliferating cells. (E) Quantification of cell proliferation by BrdU labeling index. In E15.5 ureter, p<0.001 for epithelial layer (n = 20) and p = 0.49 for mesenchymal inner layer (n = 20). In distal segment of E16.5 ureter, p<0.001 for epithelial layer (n = 20) and p = 0.59 for mesenchymal inner layer (n = 20). (F, G) BrdU incorporation analysis of epithelial cell proliferation on transverse section of cultured E15.0 ureters treated with conditioned media transfected either with pcDNA3.1 vector (Mock) (F) or an Fstl1 overexpress plasmid (G). Arrows point to representative proliferating cells. (H) HEK293 cells were transfected with a plasmid expressing mouse Fstl1 protein or pcDNA3.1 (mock). Western blot analysis using an anti-Fstl1 antibody indicated that Fstl1 was overexpressed in both the cultured media and cell pellet. (I) Quantification of cell proliferation by BrdU labeling index in the cultured E15.0 ureteric epithelium. p<0.005 (n  = 7). Scale bar: (A-D, F-G) 20µm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0032554), licensed under a CC-BY license. Not internally tested by R&D Systems.
Proliferation defects in Fstl1-/- ureteric epithelial cells.(A-D) Analysis of cell proliferation on transverse sections of wild-type (A, C) and Fstl1-/- (B, D) ureters by BrdU incorporation at E15.5 (A, B) and E16.5 ...read more
Proliferation defects in Fstl1-/- ureteric epithelial cells.(A-D) Analysis of cell proliferation on transverse sections of wild-type (A, C) and Fstl1-/- (B, D) ureters by BrdU incorporation at E15.5 (A, B) and E16.5 ...read more
Endogenous Fstl1 does not contribute to TGF‐ beta 1/Smad 2/3 signaling or myofibroblast transdifferentiationFstl1 ablation by siRNA Fstl1 (6 pmol) in NRCFbs. siRNA Fstl1 or siRNA non‐targeting negative control was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1738
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Catalog# & Formulation Size Price
Carrier Free
AF1738-SP
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Follistatin-like 1/FSTL1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-like 1/FSTL1
Glu19-Ile306
Accession # Q62356
Specificity
Detects mouse Follistatin-like 1/FSTL1 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Follistatin-like 1/FSTL1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FSTL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1738 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Follistatin-like 1/FSTL1 Antibody [Unconjugated]

  • Flik
  • FLJ50214
  • Follistatin-like 1
  • Follistatin-like protein 1
  • follistatin-related protein 1
  • Follistatin-related Protein
  • FRP
  • FRPFLJ52277
  • FSL1
  • FSTL1
  • TSC-36

Background

Mouse FSTL1, also known as FSL1 and follistatin-related protein (FRP), is a secreted protein that contains a follistatin-like domain, a Kazal-like domain and a von Willebrand factor C domain. Human FSTL1 shares 92% amino acid sequence identity with the mouse FSTL1, also known as TSC-36.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Follistatin-like 1/FSTL1 Antibody [Unconjugated]
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Publications for Follistatin-like 1/FSTL1 Antibody (AF1738)(20)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


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ICC
(1)
IHC
(4)
IHC-Fr
(1)
IHC-P
(2)
Immunoprecipitation
(2)
Western Blot
(7)
All Applications
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Mouse
(12)
Rat
(1)
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Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using AF1738 Applications Species
Ying Yang, Tao Lu, Xiaowei Jia, Yan Gao FSTL1 Suppresses Triple-Negative Breast Cancer Lung Metastasis by Inhibiting M2-like Tumor-Associated Macrophage Recruitment toward the Lungs Diagnostics (Basel) 5/12/2023 [PMID: 37238210]
Imam KMSU, Tian Y, Xin F et al. Lactucin, a Bitter Sesquiterpene from Cichorium intybus, Inhibits Cancer Cell Proliferation by Downregulating the MAPK and Central Carbon Metabolism Pathway Molecules (Basel, Switzerland) 10/29/2022 [PMID: 36364182]
Y Zhang, Y Wang, G Zheng, Y Liu, J Li, H Huang, C Xu, Y Zeng, X Zhang, J Qin, C Dai, HO Hambrock, U Hartmann, B Feng, KK Mak, Y Liu, HY Lan, Y Huang, ZH Zheng, Y Xia Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/beta-catenin signaling in obstructed kidneys in vivo The Journal of Biological Chemistry, 2022-05-04;0(0):102010. 2022-05-04 [PMID: 35525270] (IHC, Immunoprecipitation, Western Blot, Mouse, Rat) IHC, Immunoprecipitation, Western Blot Mouse, Rat
Z Huang, Z Zhang, Z Moazzami, R Heck, P Hu, H Nanda, K Ren, Z Sun, A Bartolomuc, Y Gao, D Chung, W Zhu, S Shen, HB Ruan Brown adipose tissue involution associated with progressive restriction in progenitor competence Cell Reports, 2022-04-12;39(2):110575. 2022-04-12 [PMID: 35417710] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
D Fang, X Shi, X Jia, C Yang, L Wang, B Du, T Lu, L Shan, Y Gao Ups and downs: the PPARgamma/p-PPARgamma seesaw of follistatin-like 1 and integrin receptor signaling in adipogenesis Molecular Metabolism, 2021-11-20;0(0):101400. 2021-11-20 [PMID: 34813964] (Western Blot, Mouse) Western Blot Mouse
Nicholas A. Maksimowski, Xuewen Song, Eun Hui Bae, Heather Reich, Rohan John, York Pei et al. Follistatin-Like-1 (FSTL1) Is a Fibroblast-Derived Growth Factor That Contributes to Progression of Chronic Kidney Disease International Journal of Molecular Sciences 9/1/2021 [PMID: 34502419]
Jie Ma, Ying Yang, Lulu Wang, Xiaowei Jia, Tao Lu, Yiyan Zeng et al. Follistatin-like 1 deficiency impairs T cell development to promote lung metastasis of triple negative breast cancer Aging (Albany NY) 3/15/2021 [PMID: 33639614]
D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury Sci Rep, 2019-11-13;9(1):16736. 2019-11-13 [PMID: 31723159] (Western Blot, Mouse) Western Blot Mouse
K Kretzschma, Y Post, M Bannier-Hé, A Mattiotti, J Drost, O Basak, VSW Li, M van den Bo, QD Gunst, D Versteeg, L Kooijman, S van der El, JH van Es, E van Rooij, MJB van den Ho, H Clevers Profiling proliferative cells and their progeny in damaged murine hearts Proc. Natl. Acad. Sci. U.S.A., 2018-12-07;0(0):. 2018-12-07 [PMID: 30530645] (IHC-Fr, IHC-P, Mouse) IHC-Fr, IHC-P Mouse
S Yamada, N Itoh, T Nagai, T Nakai, D Ibi, A Nakajima, T Nabeshima, K Yamada Innate immune activation of astrocytes impairs neurodevelopment via upregulation of follistatin-like 1 and interferon-induced transmembrane protein 3 J Neuroinflammation, 2018-10-22;15(1):295. 2018-10-22 [PMID: 30348171] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Show All 20 Publications.

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Bioinformatics

Gene Symbol FSTL1
Uniprot