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Ferredoxin Reductase Antibody

Images

 
Independent Antibodies: Western Blot: Ferredoxin Reductase Antibody [NBP2-38706] - Analysis using Anti-FDXR antibody NBP2-38706 (A) shows similar pattern to independent antibody NBP2-38530 (B).
Immunocytochemistry/ Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38706] - Staining of human cell line Hep G2 shows localization to mitochondria. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38706] - Staining in human adrenal gland and lymph node tissues using NBP2-38706 antibody. Corresponding FDXR RNA-seq data ...read more
Orthogonal Strategies: Western Blot: Ferredoxin Reductase Antibody [NBP2-38706] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: ...read more
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38706] - Staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.
Independent Antibodies: Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38706] - Staining of human adrenal gland, liver, lymph node and testis using Anti-FDXR antibody NBP2-38706 (A) shows ...read more
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38706] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38706] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38706] - Staining of human testis shows strong granular cytoplasmic positivity in Leydig cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Ferredoxin Reductase Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PFDSKLGVIPNVEGRVMDVPGLYCSGWVKRGPTGVIATTMTDSFLTGQMLLQDLKAGLLPSGPRPGYAAIQALLSSRGVRPVSFSDWEKLDAE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FDXR
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Ferredoxin Reductase Protein (NBP2-38706PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Ferredoxin Reductase Antibody

  • Adrenodoxin reductase
  • ADXREC 1.18.1.2
  • ferredoxin reductaseAR
  • Ferredoxin--NADP(+) reductase
  • NADPH:adrenodoxin oxidoreductase, mitochondrial

Background

The Ferredoxin Reductase gene encodes a mitochondrial flavoprotein that initiates electron transport for cytochromes P450 receivingelectrons from NADPH. Multiple alternatively spliced transcript variants of this gene have been described although thefull-length nature of only

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FDXR
Uniprot