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Fc gamma RIIB/CD32b Antibody (110) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
110
Clonality
Monoclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-89364AF350
Alexa Fluor 405
NBP2-89364AF405
Alexa Fluor 488
NBP2-89364AF488
Alexa Fluor 532
NBP2-89364AF532
Alexa Fluor 594
NBP2-89364AF594
Alexa Fluor 647
NBP2-89364AF647
Alexa Fluor 700
NBP2-89364AF700
Alexa Fluor 750
NBP2-89364AF750
Allophycocyanin
NBP2-89364APC
Biotin
NBP2-89364B
DyLight 350
NBP2-89364UV
DyLight 405
NBP2-89364V
DyLight 488
NBP2-89364G
DyLight 550
NBP2-89364R
DyLight 594
NBP2-89364DL594
DyLight 650
NBP2-89364C
DyLight 680
NBP2-89364FR
DyLight 755
NBP2-89364IR
FITC
NBP2-89364F
HRP
NBP2-89364H
Janelia Fluor 525
NBP2-89364JF525
Janelia Fluor 549
NBP2-89364JF549
Janelia Fluor 646
NBP2-89364JF646
Janelia Fluor 669
NBP2-89364JF669
PE
NBP2-89364PE
PerCP
NBP2-89364PCP
Unconjugated
NBP2-89364-100ul
mFluor Violet 450 SE
NBP2-89364MFV450
mFluor Violet 500 SE
NBP2-89364MFV500
mFluor Violet 610 SE
NBP2-89364MFV610
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Fc gamma RIIB/CD32b Antibody (110) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human Fc gamma RIIB/CD32b extracellular domain (Accession#: Ala 46-Pro 217; NP_001002274.1).
Specificity
Has cross-reactivity in ELISA with:

Human CD64
Human CD16b
No cross-reactivity in ELISA with:

Human CD32a
Human CD16a
Mouse FCGR2B
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
FCGR2B
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Fc gamma RIIB/CD32b Antibody (110) [CoraFluor™ 1]

  • CD32 antigen
  • CD32b
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RIIB
  • FCG2
  • Fc-gamma RII-b
  • fc-gamma-RIIb
  • FCGR2
  • FCGR2B
  • FcgRIIB
  • FCRIIB
  • fcRII-b
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FCGR2B