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Fc gamma RII/CD32 Antibody

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Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Staining (8ug/ml) of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.
Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.
Immunocytochemistry/ Immunofluorescence: Goat Polyclonal Fc gamma RII/CD32 Antibody [NB100-1532] - Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Fc gamma RII/CD32 Antibody Summary

Immunogen
Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus according to NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1.
Epitope
C-PDALEEPDDQNRI
Specificity
This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FCGR2B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:32000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 6 - 8 ug/ml
  • Immunohistochemistry-Paraffin 6 - 8 ug/ml
  • Western Blot 0.3-1 ug/ml
Application Notes
WB: Approx 38 kDa band observed in Daudi lysates (calculated MW of 34.0 kDa according to NP_003992.3).
Control
K-562 Whole Cell Lysate
Fc gamma RIIB/CD32b Overexpression Lysate
Publications
Read Publications using NB100-1532.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fc gamma RII/CD32 Antibody

  • CD32 antigen
  • CD32B
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RII
  • Fc gamma RIIb
  • FCG2
  • Fc-gamma RII-b
  • FcgammaRIIb
  • fc-gamma-RIIb
  • FCGR2
  • FcgRII
  • FcgRIIB
  • fcRII-b
  • FcRII-c
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RII/CD32 Antibody (NB100-1532)(2)


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

K-562 Whole Cell Lysate
Fc gamma RIIB/CD32b Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FCGR2B
Entrez
Uniprot