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FAIM1 Antibody

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Western Blot: FAIM1 Antibody [NB100-77331] - Analysis of FAIM1 using NB100-77331 at 0.5ug/ml in Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: FAIM1 Antibody [NB100-77331] - Analysis of FAIM1 using NB100-77331 at 0.5ug/ml in Human Lymph Node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FAIM1 Antibody [NB100-77331] - (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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FAIM1 Antibody Summary

Immunogen
Peptide with sequence C-KKYMEDRSKTTN corresponding to internal region according to NP_001028203.1, NP_001028204.1, NP_060617.1.
Epitope
KKYMEDRSKTTN
Specificity
This antibody is expected to recognise all reported isoforms (NP_001028202.1; NP_001028203.1; NP_001028204.1; NP_060617.1).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FAIM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human lymph node lysates (calculated MW of 24.0 kDa band according to NP_001028202.1). IHC-P: Human placenta shows cytoplasm staining of trophoblasts.
Control
FAIM1 Overexpression Lysate
Publications
Read Publication using NB100-77331.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FAIM1 Antibody

  • FAIM1FLJ10582
  • fas apoptotic inhibitory molecule 1
  • Fas apoptotic inhibitory molecule

Background

FAIM1 (or Fas Apoptotic Inhibitory Molecule 1) is a recently discovered negative regulator of apoptosis. FAIM1 has no significant regions of homology to other gene products that modulate Fas killing. It plays a role as an inducible effector molecule that mediates Fas resistance produced by surface Ig engagement in B cells. Overexpression of FAIM1 diminishes sensitivity to Fas-mediated apoptosis of B and non-B cell lines. FAIM1 is highly evolutionarily conserved and is widely expressed in murine tissues, suggesting that FAIM1 plays an important role in cellular physiology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FAIM1 Antibody (NB100-77331)(1)


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Product General Protocols

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Video Protocols

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Control Lysate(s)

FAIM1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FAIM
Entrez
Uniprot