Home » FABP5/E-FABP » FABP5 Antibodies » FABP5/E-FABP Antibody (311215) [Unconjugated]
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FABP5/E-FABP Antibody (311215) [Unconjugated]

 (7 Publications)
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Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E-FABP Monoclonal Antibody (Catalog # MAB3077) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP5/ E-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human FABP5/E-FABP Monoclonal Antibody (Catalog # MAB3077) ...read more
FABP5 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human FABP5 Monoclonal Antibody (Catalog # MAB3077) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF, KO
Clone
311215
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3077U-100UG
Alexa Fluor 405
FAB3077V-100UG
Alexa Fluor 488
FAB3077G-100UG
Alexa Fluor 532
FAB3077X
Alexa Fluor 594
FAB3077T-100UG
Alexa Fluor 647
FAB3077R-100UG
Alexa Fluor 700
FAB3077N-100UG
Alexa Fluor 750
FAB3077S-100UG
Allophycocyanin
FAB3077A
Allophycocyanin/Cy7
FAB3077APCCY7
Biotin
FAB3077B
CoraFluor 1
FAB3077CL1
DyLight 350
FAB3077D
DyLight 405
FAB3077E
DyLight 488
FAB3077K
DyLight 550
FAB3077L
DyLight 594
FAB3077M
DyLight 650
FAB3077W
DyLight 680
FAB3077Y
DyLight 755
FAB3077Z
FITC
FAB3077F
HRP
FAB3077H
Janelia Fluor 525
FAB3077JF525
Janelia Fluor 549
FAB3077I
Janelia Fluor 635
FAB3077JF635
Janelia Fluor 646
FAB3077J
Janelia Fluor 669
FAB3077JF669
PE
FAB3077P
PE/Atto594
FAB3077PEATT594
PE/Cy5.5
FAB3077PECY55
PE/Cy7
FAB3077PECY7
PerCP
FAB3077C
mFluor Violet 450 SE
FAB3077MFV450
mFluor Violet 500 SE
FAB3077MFV500
mFluor Violet 610 SE
FAB3077MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3077-SP
Carrier Free
FAB3077V-100UG
Carrier Free
FAB3077U-100UG
Carrier Free
FAB3077T-100UG
Carrier Free
FAB3077S-100UG
Carrier Free
FAB3077R-100UG
Carrier Free
FAB3077N-100UG
Carrier Free
FAB3077G-100UG
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FABP5/E-FABP Antibody (311215) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FABP5/E‑FABP
Ala2-Glu135
Accession # Q01469
Specificity
Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FABP1, 2, 3, 4, 6, 7, or 9 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FABP5
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB3077 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP5/E-FABP Antibody (311215) [Unconjugated]

  • EFABP
  • E-FABP
  • E-FABPPAFABP
  • FABP5
  • fatty acid binding protein 5 (psoriasis-associated)
  • Fatty acid-binding protein 5
  • PA-FABPepidermal
  • Psoriasis-associated fatty acid-binding protein homolog

Background

FABP5, also known as epidermal fatty acid binding protein (E-FABP), is expressed in skin, lens, adipose tissue, endothelial cells, heart, brain and placenta. FABP-5 is associated with keratinocytes and adipocytes, and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors.  Human FABP-5 shares 80%, 81%, and 92% aa identity with mouse, rat and bovine FABP-5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP5/E-FABP Antibody (MAB3077)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, IHC, IHC-P, Western Blot.


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Flow Cytometry
(1)
IHC
(1)
IHC-P
(1)
Western Blot
(2)
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Human
(4)
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Showing Publications 1 - 7 of 7.
Publications using MAB3077 Applications Species
CS Hughes, JA ChinAleong, HM Kocher CRABP2 and FABP5 expression levels in diseased and normal pancreas Ann Diagn Pathol, 2020-06-20;47(0):151557. 2020-06-20 [PMID: 32593808] (IHC, Human) IHC Human
D Rao, AL Perraud, C Schmitz, F Gally Cigarette smoke inhibits LPS-induced FABP5 expression by preventing c-Jun binding to the FABP5 promoter PLoS ONE, 2017-05-18;12(5):e0178021. 2017-05-18 [PMID: 28542209] (Western Blot, Human) Western Blot Human
Wentao Chen, Jiajia Dong, Lars Plate, David E. Mortenson, Gabriel J. Brighty, Suhua Li et al. Arylfluorosulfates Inactivate Intracellular Lipid Binding Protein(s) through Chemoselective SuFEx Reaction with a Binding Site Tyr Residue Journal of the American Chemical Society 2016-06-15 [PMID: 27191344]
YOSHITO OHYAMA, YUKIHIRO KAWAMOTO, TADASHIGE CHIBA, KENTARO KIKUCHI, HIDEAKI SAKASHITA, KAZUSHI IMAI Differential expression of fatty acid-binding proteins and pathological implications in the progression of tongue carcinoma Molecular and Clinical Oncology 2014-01-01 [PMID: 24649302]
Fabienne Gally, Hong Wei Chu, Russell P. Bowler Cigarette Smoke Decreases Airway Epithelial FABP5 Expression and Promotes Pseudomonas aeruginosa Infection PLoS ONE 2013-01-22 [PMID: 23349676]
Gupta S, Pramanik D, Mukherjee R, Campbell NR, Elumalai S, de Wilde RF, Hong SM, Goggins MG, De Jesus-Acosta A, Laheru D, Maitra A Molecular determinants of retinoic acid sensitivity in pancreatic cancer. Clin. Cancer Res., 2011-10-18;18(1):280-9. 2011-10-18 [PMID: 22010213] (IHC-P, Western Blot, Human) IHC-P, Western Blot Human
Liebertz DJ, Lechner MG, Masood R Establishment and characterization of a novel head and neck squamous cell carcinoma cell line USC-HN1. Head Neck Oncol, 2010-02-22;2(0):5. 2010-02-22 [PMID: 20175927] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol FABP5
Uniprot