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Exosome component 10 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Exosome component 10 Antibody [NBP1-82447] - Staining of human cell line U-2 OS shows localization to nucleus, nucleoli and cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-82447] - Staining of human pancreas shows moderate nucleolar positivity which was combined with distinct cytoplasmic staining in intercalated ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Exosome component 10 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FAHPYQYELNHFTPADAVLQKPQPQLYRPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EXOSC10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Exosome component 10 Protein (NBP1-82447PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Exosome component 10 Antibody

  • autoantigen PM-SCL
  • EC 3.1.13.-
  • exosome component 10
  • P100 polymyositis-scleroderma overlap syndrome-associated autoantigen
  • p2
  • p3
  • p4
  • PM/Scl-100Autoantigen PM/Scl 2
  • PM-Scl
  • PMSCL2PMSCL
  • polymyositis/scleroderma autoantigen 2 (100kD)
  • Polymyositis/scleroderma autoantigen 2
  • polymyositis/scleroderma autoantigen 2, 100kDa
  • RRP6
  • Rrp6p
  • RRP6Polymyositis/scleroderma autoantigen 100 kDa

Background

Part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. Required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Could be an exonuclease . Plays a role in replication-dependent histone mRNA degradation. Mediates the association of MTR4, C1D and MPP6 wth the exosome and this complex is required for the maturation of 5.8S rRNA. Required for nucleolar localization of C1D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Exosome component 10 Antibody (NBP1-82447). (Showing 1 - 1 of 1 FAQ).

  1. I'm wondering whether this EXOSC10 Antibody (NBP1-82447) also works for yeast RRP6. Have you guys ever tested that? The reason I'm asking is that the immunogen used (FAHPYQYELNHFTPADAVLQKPQPQLYRPIEETPCHFISSLDELVELNEKLLNCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDF) to raise this Ab contains conserved regions between EXOSC10 and yRRP6.
    • We have not tested this antibody for yeast RRP6. However if you would like to test this we can offer you our We have not tested NB600-582 with any dog samples, and cannot guarantee that it will cross-react. However, if you are interested in trying this antibody with dog samples you would be eligible for our Innovators Reward Program. We offer this reward for testing an anitbody with an application or species that we have not yet tested.
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Bioinformatics

Gene Symbol EXOSC10