Novus Biologicals products are now on bio-techne.com

ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free

Images

 
Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 ...read more
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal ...read more
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human ESR1(NP_000116) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ESR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free

  • ER alpha
  • ER
  • Era
  • ER-alpha
  • ESR1
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor alpha
  • estrogen receptor
  • NR3A1
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
DY1707
Species: Hu
Applications: ELISA
NB200-305
Species: Hu, Pm, Mu, Rb
Applications: ChIP, DB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
1129-ER
Species: Hu
Applications: BA
H00005555-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF5739
Species: Hu, Mu, Rt
Applications: WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
AF231
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
NB100-1596
Species: Hu, Mu, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF262
Species: Hu
Applications: ICC, IHC, Neut, WB
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-38530
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
NBP2-70687
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for ER alpha/NR3A1 Antibody (NBP2-70687) (0)

There are no publications for ER alpha/NR3A1 Antibody (NBP2-70687).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ER alpha/NR3A1 Antibody (NBP2-70687) (0)

There are no reviews for ER alpha/NR3A1 Antibody (NBP2-70687). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ER alpha/NR3A1 Antibody (NBP2-70687) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol ESR1