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ER alpha/NR3A1 Antibody (EVG F9)

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Analysis of various whole cell lysates.
Analysis of various whole cell lysates.
Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in U251 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and ...read more
Cancer biopsies of deparaffinized Human breast carcinoma tissues.
Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in Hela Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and ...read more
Analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) shows staining in MCF-7 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and ...read more
Normal deparaffinized Human uterus tissue.

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
EVG F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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ER alpha/NR3A1 Antibody (EVG F9) Summary

Immunogen
Mix of synthetic peptides corresponding to residuesT(140) V R E A G P P A F Y R P N S(154) & E(247) V G M M K G G I R K D R R G G R(263) of human ER alpha.
Specificity
Estrogen Receptor alpha (EVG F9)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ESR1
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500 - 1:2000
Application Notes
WB: Detects an approx. 68 kDa protein representing estrogen receptor alpha in MCF-7 cells, human breast tumors, and rabbit vaginal tissue.
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 9582378).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for ER alpha/NR3A1 Antibody (EVG F9)

  • ER alpha
  • ER
  • Era
  • ER-alpha
  • ESR1
  • ESRESRA
  • Estradiol receptor
  • estrogen receptor 1
  • estrogen receptor alpha delta 3*4,56,7*/819-2 isoform
  • estrogen receptor alpha delta 4 +49 isoform
  • estrogen receptor alpha delta 4*5,6,7*/654 isoform
  • estrogen receptor alpha
  • estrogen receptor
  • NR3A1
  • NR3A1DKFZp686N23123
  • Nuclear receptor subfamily 3 group A member 1

Background

Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ESR1
Entrez
Uniprot