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eNOS Antibody (6H2) - Azide and BSA Free

Images

 
Western Blot: eNOS Antibody (6H2) - Azide and BSA Free [NBP2-80720] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - Azide and BSA Free [NBP2-80720] - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with ...read more
Immunohistochemistry: eNOS Antibody (6H2) - Azide and BSA Free [NBP2-80720] - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: eNOS Antibody (6H2) - Azide and BSA Free [NBP2-80720] - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
6H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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eNOS Antibody (6H2) - Azide and BSA Free Summary

Immunogen
Purified recombinant fragment of human eNOS expressed in E. coli. [UniProt# P29474]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOS3
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 2 ug/ml
Application Notes
This eNOS (6H2) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for eNOS Antibody (6H2) - Azide and BSA Free

  • Constitutive NOS
  • EC 1.14.13.39
  • ECNOS
  • Endothelial NOS
  • endothelial
  • eNOS
  • eNOScNOS
  • nitric oxide synthase 3 (endothelial cell)
  • NOS type III
  • NOS3
  • NOSIII

Background

eNOS (Endothelial nitric-oxide synthase), also known as NOS3, it is an important enzyme in the cardiovascular system. It is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NOS3