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ELF3/ESE-1 Antibody (662533) [Unconjugated]

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ELF3 Monoclonal Antibody (Catalog # MAB5787) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and A549 human lung ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
662533
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5787-SP
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ELF3/ESE-1 Antibody (662533) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ELF3
Met1-Gly173
Accession # P78545
Specificity
Detects human and mouse ELF3 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ELF3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ELF3/ESE-1 Antibody (662533) [Unconjugated]

  • E74-like factor 3 (ets domain transcription factor, epithelial-specific )
  • E74-like factor 3
  • ELF3
  • Epithelial-restricted with serine box
  • epithelial-specific)
  • Epithelium-restricted Ets protein ESX
  • Epithelium-specific Ets transcription factor 1
  • EPR1
  • EPR-1
  • ERT
  • ESX
  • ets domain transcription factor, serine box (epithelial-specific)
  • ETS-related transcription factor Elf-3
  • JEN
  • serine box

Background

ELF3 (E74-Like Factor 3), also known as ESE-1, ESX, ERT and JEN, is a 41-43 kDa member of the ETS family of proteins.  During uncomplicated (non-inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation.  Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes express ELF3 and produce molecules such as Ang1 and COX2.  Human ELF3 is 371 amino acids (aa) in length.  It contains one PNT/pointed dimerization domain (aa 46-132), a protein stabilizing PEST sequence (aa 210-225), an A/T Hook region that binds to AT-rich DNA sequences (aa 236-252), and an ETS DNA binding domain (aa 273-355).  ELF3 interacts with CREBBP, EP300, KU70 and KU86.  There is one splice variant that shows a deletion of aa 174-200.  Over aa 1-173, human ELF3 shares 87% aa identity with mouse ELF3.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELF3/ESE-1
Antibody (662533) [Un ...
MAB5787
ELF3/ESE-1 Antibody (662533) [Unconjugated]
Species: Hu, Mu
Applications: WB

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Bioinformatics

Gene Symbol ELF3
Uniprot