![Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rSPM217) - Azide and BSA Free [NBP3-08403] - Formalin-fixed, paraffin-embedded human liver stained with ECM-1/Secretory Component P85 Recombinant Mouse Monoclonal Antibody (rSPM217).](http://images.novusbio.com/fullsize/ECM-1-Secretory-Component-P85-Antibody-rSPM217-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08403-img0001.jpg "Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rSPM217) - Azide and BSA Free [NBP3-08403] - Formalin-fixed, paraffin-embedded human liver stained with ECM-1/Secretory Component P85 Recombinant Mouse Monoclonal Antibody (rSPM217).")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | rSPM217 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07282). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant full-length human ECM-1/Secretory Component P85 protein (Uniprot: Q16610) |
| Localization | Cell surface and cytoplasmic |
| Specificity | This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ECM1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ECM-1/Secretory Component P85 Antibody (NBP3-08403)Find related products by research area.
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| Gene Symbol | ECM1 |
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