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EBI3 Antibody (607215) [Unconjugated]

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Recombinant Human IL‑39 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL‑23 p19 Monoclonal Antibody (MAB17161) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human EBI3 Monoclonal Antibody (Catalog # MAB64561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Recombinant Human IL‑39 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL‑23 p19 Monoclonal Antibody (MAB17161) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human EBI3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
607215
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB64561-SP
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EBI3 Antibody (607215) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EBI3
Arg21-Lys229
Accession # Q14213
Specificity
Detects human EBI3 in ELISAs for IL-39 detection.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBI3 Antibody (607215) [Unconjugated]

  • cytokine receptor
  • EBI3
  • EBI-3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 beta
  • IL-27 subunit beta
  • IL27B
  • IL-27B
  • IL35 EBI3
  • IL-35 EBI3
  • interleukin-27 subunit beta

Background

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EBI3 Antibody
(607215) [Unconjugate
MAB64561
EBI3 Antibody (607215) [Unconjugated]
Species: Hu
Applications: ELISA

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