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EBI3 Antibody (355022) [Phycoerythrin]

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Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit PE-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control antibody (Catalog # IC006P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Mouse splenocytes untreated (open histogram) or treated with LPS (filled histogram) were stained with Rat Anti-Mouse IL-27/IL-35 EBI3 Subunit PE-conjugated Monoclonal Antibody (Catalog # IC18341A) or isotype control ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
355022
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
IC18341A
PerCP (Peridinin-chlorophyll Protein Complex)
IC18341C
Unconjugated
MAB18341
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB18341
Carrier Free
IC18341C
Carrier Free
MAB18341
Carrier Free
IC18341A
Carrier Free
MAB18341-SP
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EBI3 Antibody (355022) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑27 heterodimer
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Specificity
Detects mouse IL-27/IL-35 EBI3 Subunit in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human IL‑27/IL-35 EBI3 subunit.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
EBI3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC18341P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EBI3 Antibody (355022) [Phycoerythrin]

  • cytokine receptor
  • EBI3
  • EBI-3
  • EBV-induced gene 3 protein
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • Epstein-Barr virus-induced gene 3 protein
  • IL-27 beta
  • IL-27 subunit beta
  • IL27B
  • IL-27B
  • IL35 EBI3
  • IL-35 EBI3
  • interleukin-27 subunit beta

Background

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide anda 209 aa mature protein with two fibronectin type III domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EBI3 Antibody
(355022) [Phycoerythr
IC18341P
EBI3 Antibody (355022) [Phycoerythrin]
Species: Mu
Applications: Flow

Publications for EBI3 Antibody (IC18341P)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(2)
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Mouse
(2)
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Bioinformatics

Gene Symbol EBI3
Uniprot