![Western Blot: Dynamin 2 Antibody [NB300-617] - Western blot analysis of Dynamin II was performed using whole cell lysates and tissue lysate of SH-SY5Y (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), SK-N-AS (Lane 4), U- 87 MG (Lane 5) and Rat Brain (Lane 6). The blot was probed with Anti-Dynamin II Rabbit polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) )Secondary Antibody, HRP conjugate. A band ~98 kDa corresponding to Dynamin II was observed across cell lines and tissue lysate tested. A non specific band of ~48 KDa was observed across cell lines and tissue lysate tested except for Neuro-2a cell line.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NB300-617-img0006.jpg "Western Blot: Dynamin 2 Antibody [NB300-617] - Western blot analysis of Dynamin II was performed using whole cell lysates and tissue lysate of SH-SY5Y (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), SK-N-AS (Lane 4), U- 87 MG (Lane 5) and Rat Brain (Lane 6). The blot was probed with Anti-Dynamin II Rabbit polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) )Secondary Antibody, HRP conjugate. A band ~98 kDa corresponding to Dynamin II was observed across cell lines and tissue lysate tested. A non specific band of ~48 KDa was observed across cell lines and tissue lysate tested except for Neuro-2a cell line.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic Peptide: C S(760) P T P Q R R P V S S I H P P G R P P A(779) |
| Specificity | Dynamin 2 - Synapse |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DNM2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Detects an approx. 100 kDa protein representing Dynamin 2 from HeLa cell lysate. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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EEA1 Antibodies in Endosomal Transport and Signalling Studies EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have ... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Trito(TM) X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Recombinant Secondary Antibody, Alexa Fluor(R) 488 conjugate, for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong(TM) Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing Cytoskeletal and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-617-img0004.jpg "Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Trito(TM) X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Recombinant Secondary Antibody, Alexa Fluor(R) 488 conjugate, for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong(TM) Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing Cytoskeletal and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background.")
![Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin II was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin II Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin . Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-NB300-617-img0005.jpg "Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin II was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin II Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin . Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.")
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