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DPM3 Antibody

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Western Blot: DPM3 Antibody [NBP1-81216] - Analysis in control (vector only transfected HEK293T lysate) and DPM3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: DPM3 Antibody [NBP1-81216] -Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells and weak cytoplasmic positivity in endocrine glandular cells.
Immunohistochemistry-Paraffin: DPM3 Antibody [NBP1-81216] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: DPM3 Antibody [NBP1-81216] -Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: DPM3 Antibody [NBP1-81216] -Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and leydig cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DPM3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FHDCEDAARELQSQIQEARADLARR
Predicted Species
Mouse (96%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DPM3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
DPM3 Protein (NBP1-81216PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DPM3 Antibody

  • CDG1O
  • Dolichol-phosphate mannose synthase subunit 3
  • dolichol-phosphate mannosyltransferase subunit 3
  • Dolichyl-phosphate beta-D-mannosyltransferase subunit 3
  • dolichyl-phosphate mannosyltransferase polypeptide 3
  • DPM synthase complex subunit 3
  • Mannose-P-dolichol synthase subunit 3
  • MGC125904
  • MGC125905
  • MGC34275
  • MPD synthase subunit 3
  • prostin 1
  • prostin-1

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a subunit of dolichyl-phosphate mannosyltransferase and acts as a stabilizer subunit of the dolichyl-phosphate mannosyltransferase complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

FAQs for DPM3 Antibody (NBP1-81216). (Showing 1 - 1 of 1 FAQ).

  1. I'm interested in NBP1-81216. Since I will be using it on zebrafish protein extracts, I'd like to know if the epitope recognised by the antibody is conserved?
    • The epitope information is considered propriatary so I have carried out a blast. The % homology between the human protein and the zebrafish protein is approximately 57% so I do not recommend use. Our lab only recommends use if the % homology is 85% or above.
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Bioinformatics

Gene Symbol DPM3
Entrez