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Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence)

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ELISA: Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence) [NBP2-67931] - Samples were spiked with high concentrations of Human Dopa Decarboxylase/DDC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence) [NBP2-67931] - Samples were spiked with high concentrations of Human Dopa Decarboxylase/DDC and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence) [NBP2-67931] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

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Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Human DDC in samples. No significant cross-reactivity or interference between Human DDC and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 8.19%
Intra-Assay
CV% < 9.28%
Spike Recovery
92-116%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
DDC

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence)

  • AADC
  • AADCaromatic-L-amino-acid decarboxylase
  • DDC
  • dopa decarboxylase (aromatic L-amino acid decarboxylase)
  • Dopa Decarboxylase
  • EC 4.1.1
  • EC 4.1.1.28

Background

DOPA Decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Dopa
Decarboxylase/DDC ELI ...
NBP2-67931
Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence)
Species: Hu
Applications: ELISA

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Product PDFs

  Human Dopa Decarboxylase/DDC ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol DDC